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  In vivo time-lapse imaging shows diverse niche engagement by quiescent and naturally activated hematopoietic stem cells

Rashidi, N. M., Scott, M. K., Scherf, N., Krinner, A., Kalchschmidt, J. S., Gounaris, K., et al. (2014). In vivo time-lapse imaging shows diverse niche engagement by quiescent and naturally activated hematopoietic stem cells. Blood, 124(1), 79-83. doi:10.1182/blood-2013-10-534859.

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 Creators:
Rashidi, Narges M.1, Author
Scott, Mark K.1, Author
Scherf, Nico1, Author              
Krinner, Axel1, Author
Kalchschmidt, Jens S.1, Author
Gounaris, Kleoniki1, Author
Selkirk, Murray E.1, Author
Roeder, Ingo1, Author
Lo Celso, Cristina1, Author
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1External Organizations, ou_persistent22              

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Free keywords: Antagonists; cd44 antigens; cxcr4 receptors; Diagnostic imaging; Hematopoietic stem cells; Homing-associated cell adhesion molecule; Infections; Mice; Trichinella spiralis; Bone marrow
 Abstract: Hematopoietic stem cells (HSCs) maintain the turnover of mature blood cells during steady state and in response to systemic perturbations such as infections. Their function critically depends on complex signal exchanges with the bone marrow (BM) microenvironment in which they reside, but the cellular mechanisms involved in HSC-niche interactions and regulating HSC function in vivo remain elusive. We used a natural mouse parasite, Trichinella spiralis, and multipoint intravital time-lapse confocal microscopy of mouse calvarium BM to test whether HSC-niche interactions may change when hematopoiesis is perturbed. We find that steady-state HSCs stably engage confined niches in the BM whereas HSCs harvested during acute infection are motile and therefore interact with larger niches. These changes are accompanied by increased long-term repopulation ability and expression of CD44 and CXCR4. Administration of a CXCR4 antagonist affects the duration of HSC-niche interactions. These findings suggest that HSC-niche interactions may be modulated during infection.

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Language(s): eng - English
 Dates: 2013-10-252014-04-282014-05-212014-07-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1182/blood-2013-10-534859
PMID: 24850759
PMC: PMC4125355
 Degree: -

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Title: Blood
  Other : Blood
Source Genre: Journal
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Publ. Info: Philadelphia, Pa. : W.B. Saunders
Pages: - Volume / Issue: 124 (1) Sequence Number: - Start / End Page: 79 - 83 Identifier: ISSN: 0006-4971
CoNE: https://pure.mpg.de/cone/journals/resource/954925385125