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  Quantitative analysis of astrogliosis in drug-dependent humans

Weber, M., Scherf, N., Kahl, T., Braumann, U.-D., Scheibe, P., Kuska, J.-P., et al. (2013). Quantitative analysis of astrogliosis in drug-dependent humans. Brain Research, 1500, 72-87. doi:10.1016/j.brainres.2012.12.048.

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Genre: Journal Article

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 Creators:
Weber, Marco1, Author
Scherf, Nico1, Author              
Kahl, Thomas1, Author
Braumann, Ulf-Dietrich1, Author
Scheibe, Patrick1, Author
Kuska, Jens-Peer1, Author
Bayer, Ronny1, Author
Büttner, Andreas1, Author
Franke, Heike1, Author
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1External Organizations, ou_persistent22              

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Free keywords: Addiction; Astrocyte; Drug abuse; GFAP; Heroin; Quantitative image analysis; Astrogliosis
 Abstract: Drug addiction is a chronic, relapsing disease caused by neurochemical and molecular changes in the brain. In this human autopsy study qualitative and quantitative changes of glial fibrillary acidic protein (GFAP)-positive astrocytes in the hippocampus of 26 lethally intoxicated drug addicts and 35 matched controls are described. The morphological characterization of these cells reflected alterations representative for astrogliosis. But, neither quantification of GFAP-positive cells nor the Western blot analysis indicated statistical significant differences between drug fatalities versus controls. However, by semi-quantitative scoring a significant shift towards higher numbers of activated astrocytes in the drug group was detected. To assess morphological changes quantitatively, graph-based representations of astrocyte morphology were obtained from single cell images captured by confocal laser scanning microscopy. Their underlying structures were used to quantify changes in astroglial fibers in an automated fashion. This morphometric analysis yielded significant differences between the investigated groups for four different measures of fiber characteristics (Euclidean distance, graph distance, number of graph elements, fiber skeleton distance), indicating that, e.g., astrocytes in drug addicts on average exhibit significant elongation of fiber structures as well as two-fold increase in GFAP-positive fibers as compared with those in controls. In conclusion, the present data show characteristic differences in morphology of hippocampal astrocytes in drug addicts versus controls and further supports the involvement of astrocytes in human pathophysiology of drug addiction. The automated quantification of astrocyte morphologies provides a novel, testable way to assess the fiber structures in a quantitative manner as opposed to standard, qualitative descriptions.

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Language(s): eng - English
 Dates: 2012-12-232013-01-182013-03-15
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.brainres.2012.12.048
PMID: 23337617
 Degree: -

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Title: Brain Research
  Other : Brain Res.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 1500 Sequence Number: - Start / End Page: 72 - 87 Identifier: ISSN: 0006-8993
CoNE: https://pure.mpg.de/cone/journals/resource/954926250616