English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics

Schmid, B., Shah, G., Scherf, N., Weber, M., Thierbach, K., Campos, C. P., et al. (2013). High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics. Nature Communications, 4: 2207. doi:10.1038/ncomms3207.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Schmid, Benjamin1, Author
Shah, Gopi1, Author
Scherf, Nico1, Author              
Weber, Michael1, Author
Thierbach, Konstantin1, Author
Campos, Citlali Pérez1, Author
Roeder, Ingo1, Author
Aanstad, Pia1, Author
Huisken, Jan1, Author
Affiliations:
1External Organizations, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: The ever-increasing speed and resolution of modern microscopes make the storage and post-processing of images challenging and prevent thorough statistical analyses in developmental biology. Here, instead of deploying massive storage and computing power, we exploit the spherical geometry of zebrafish embryos by computing a radial maximum intensity projection in real time with a 240-fold reduction in data rate. In our four-lens selective plane illumination microscope (SPIM) setup the development of multiple embryos is recorded in parallel and a map of all labelled cells is obtained for each embryo in <10 s. In these panoramic projections, cell segmentation and flow analysis reveal characteristic migration patterns and global tissue remodelling in the early endoderm. Merging data from many samples uncover stereotypic patterns that are fundamental to endoderm development in every embryo. We demonstrate that processing and compressing raw image data in real time is not only efficient but indispensable for image-based systems biology.

Details

show
hide
Language(s): eng - English
 Dates: 2013-01-082013-06-282013-07-252013
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1038/ncomms3207
PMID: 23884240
PMC: PMC3731668
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 4 Sequence Number: 2207 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723