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  High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics

Schmid, B., Shah, G., Scherf, N., Weber, M., Thierbach, K., Campos, C. P., et al. (2013). High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics. Nature Communications, 4: 2207. doi:10.1038/ncomms3207.

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Schmid, Benjamin1, Autor
Shah, Gopi1, Autor
Scherf, Nico1, Autor           
Weber, Michael1, Autor
Thierbach, Konstantin1, Autor
Campos, Citlali Pérez1, Autor
Roeder, Ingo1, Autor
Aanstad, Pia1, Autor
Huisken, Jan1, Autor
Affiliations:
1External Organizations, ou_persistent22              

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 Zusammenfassung: The ever-increasing speed and resolution of modern microscopes make the storage and post-processing of images challenging and prevent thorough statistical analyses in developmental biology. Here, instead of deploying massive storage and computing power, we exploit the spherical geometry of zebrafish embryos by computing a radial maximum intensity projection in real time with a 240-fold reduction in data rate. In our four-lens selective plane illumination microscope (SPIM) setup the development of multiple embryos is recorded in parallel and a map of all labelled cells is obtained for each embryo in <10 s. In these panoramic projections, cell segmentation and flow analysis reveal characteristic migration patterns and global tissue remodelling in the early endoderm. Merging data from many samples uncover stereotypic patterns that are fundamental to endoderm development in every embryo. We demonstrate that processing and compressing raw image data in real time is not only efficient but indispensable for image-based systems biology.

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Sprache(n): eng - English
 Datum: 2013-01-082013-06-282013-07-252013
 Publikationsstatus: Erschienen
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 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: DOI: 10.1038/ncomms3207
PMID: 23884240
PMC: PMC3731668
 Art des Abschluß: -

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Titel: Nature Communications
  Kurztitel : Nat. Commun.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: London : Nature Publishing Group
Seiten: - Band / Heft: 4 Artikelnummer: 2207 Start- / Endseite: - Identifikator: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723