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  A combinatorial native MS and LC-MS/MS approach reveals high intrinsic phosphorylation of human Tau but minimal levels of other key modifications

Drepper, F., Biernat, J., Kaniyappan, S., Meyer, H. E., Mandelkow, E.-M., Warscheid, B., et al. (2020). A combinatorial native MS and LC-MS/MS approach reveals high intrinsic phosphorylation of human Tau but minimal levels of other key modifications. The Journal of Biological Chemistry, 295(52), 18213-18225. doi:10.1074/jbc.RA120.015882.

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1-s2.0-S0021925817506936-main.pdf (Publisher version), 3MB
 
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2020
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2020 Drepper et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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 Creators:
Drepper, Friedel1, Author
Biernat, Jacek1, Author
Kaniyappan, Senthilvelrajan1, Author
Meyer, Helmut E1, Author
Mandelkow, Eva-Maria2, Author              
Warscheid, Bettina1, Author
Mandelkow, Eckhard2, Author              
Affiliations:
1External Organizations, ou_persistent22              
2Neuronal Cytoskeleton and Alzheimer's Disease, Cooperations, Center of Advanced European Studies and Research (caesar), Max Planck Society, Ludwig-Erhard-Allee 2, 53175 Bonn, DE, ou_2173677              

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Free keywords: Tau protein, (Tau)mass spectrometry (MS), phosphorylation, Alzheimer's disease, protein aggregation, LC-MS, native mass spectrometry Headline: Protein Structure and Folding; Neurobiology
 Abstract: Abnormal changes of neuronal Tau protein, such as phosphorylation and aggregation, are considered hallmarks of cognitive deficits in Alzheimer's disease. Abnormal phosphorylation is thought to precede aggregation and therefore to promote aggregation, but the nature and extent of phosphorylation remain ill-defined. Tau contains ∼85 potential phosphorylation sites, which can be phosphorylated by various kinases because the unfolded structure of Tau makes them accessible. However, methodological limitations (e.g. in MS of phosphopeptides, or antibodies against phosphoepitopes) led to conflicting results regarding the extent of Tau phosphorylation in cells. Here we present results from a new approach based on native MS of intact Tau expressed in eukaryotic cells (Sf9). The extent of phosphorylation is heterogeneous, up to ∼20 phosphates per molecule distributed over 51 sites. The medium phosphorylated fraction Pm showed overall occupancies of ∼8 Pi (± 5) with a bell-shaped distribution; the highly phosphorylated fraction Ph had 14 Pi (± 6). The distribution of sites was highly asymmetric (with 71% of all P-sites in the C-terminal half of Tau). All sites were on Ser or Thr residues, but none were on Tyr. Other known posttranslational modifications were near or below our detection limit (e.g. acetylation, ubiquitination). These findings suggest that normal cellular Tau shows a remarkably high extent of phosphorylation, whereas other modifications are nearly absent. This implies that abnormal phosphorylations at certain sites may not affect the extent of phosphorylation significantly and do not represent hyperphosphorylation. By implication, the pathological aggregation of Tau is not likely a consequence of high phosphorylation.

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Language(s): eng - English
 Dates: 2020-12-25
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 33453828
DOI: 10.1074/jbc.RA120.015882
 Degree: -

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Title: The Journal of Biological Chemistry
  Abbreviation : J Biol Chem
Source Genre: Journal
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Publ. Info: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Pages: - Volume / Issue: 295 (52) Sequence Number: - Start / End Page: 18213 - 18225 Identifier: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826_1