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  The effect of pH at hemolysis on the reconstitution of low cation permeability in human erythrocyte ghosts

Lepke, S., & Passow, H. (1972). The effect of pH at hemolysis on the reconstitution of low cation permeability in human erythrocyte ghosts. Biochimica et Biophysica Acta-Biomembranes, 255(2), 696-702. doi:10.1016/0005-2736(72)90174-5.

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 Creators:
Lepke, Sigrid1, 2, Author           
Passow, Hermann1, 2, Author           
Affiliations:
1Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_3264817              
2Max-Planck-Institut für Molekulare Genetik, Berlin, Germany, ou_persistent22              

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 Abstract: Human red blood cells were hemolysed at 0°C in hypotonic media of varying pH (range 4.5–10.0). Subsequently the pH in the hemolysate was readjusted to 7.2 and the ghosts were incubated at 37°C for 45 min. The curve relating the yield of resealed ghosts to pH showed a maximum after hemolysis at pH 6 and a broad shoulder in the range between pH 7 and 9. The experiments suggest that during hemolysis buried ionizable groups become exposed to the ambient medium. The ability of these groups to return to their original locations within the hydrophobic interior of the membrane seems to depend on the charge which they assume during their exposure to the aqueous environment. However, alternative explanations cannot be excluded.

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Language(s): eng - English
 Dates: 1971-12-122003-01-301972-02-11
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0005-2736(72)90174-5
PMID: 5057940
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Title: Biochimica et Biophysica Acta-Biomembranes
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 255 (2) Sequence Number: - Start / End Page: 696 - 702 Identifier: ISSN: 0005-2736
CoNE: https://pure.mpg.de/cone/journals/resource/954926938702