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  Effect of P-Chloromercuribenzoate (pCMB), Ouabain and 4-Acetamido-4′ISO-Thiocyamatostilbene-2,2′-Disulfonic Acid (Sits) on Proximal Tubular Transport Processes

Ullrich, K. J., Capasso, G., Rumrich, G., & Sato, K. (1977). Effect of P-Chloromercuribenzoate (pCMB), Ouabain and 4-Acetamido-4′ISO-Thiocyamatostilbene-2,2′-Disulfonic Acid (Sits) on Proximal Tubular Transport Processes. New York, London: Plenum Press.

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Genre: Conference Paper

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 Creators:
Ullrich, Karl Julius1, Author              
Capasso, Giovambattista1, Author              
Rumrich, Gerhard1, Author              
Sato, Kenzo1, Author              
Affiliations:
1Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              

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 Abstract: Using microperfusion techniques the following transport parameters of the proximal tubule were measured: 1. Isotonic fluid (Na+) absorption (JNa). 2. Zero net flux concentration (electrochemical potential) differences which are proportional to the respective active transport rates of H+ Cglycodiazine), D-glucose (α-methyl-D-glycoside), L-histidine, inorganic phosphate and calcium ions. 3. Transtubular and transcellular electrical potential differences and transcellular resistances. The following was found: 1. Ouabain (1mM) applied peritubularly in golden hamsters inhibited JNa incompletely and the sodium-coupled (secondary active) transport processes of glucose, histidine, phosphate and Ca++ by more than 80%. The H+ (glycodiazine) transport was not affected. Ouabain (1mM) plus acetazolamide (0.2 mM) inhibited JNa completely. 2. In the rat, pCMB (0.2 mM) when applied long enough, inhibits JNa completely. At a time of pCMB application when JNa is reduced to 1/3, this substance inhibits the active H+ (glycodiazine) transport which must be considered to be a direct action of pCMB. Furthermore it inhibits the secondary active phosphate transport either directly or via the inhibition of Na+ and/or H+ transport. The secondary active glucose, histidine and Ca++ transport are little affected by pCMB. pCMB reduces the cell potential, reversibly, yet leaves unchanged the resistance ratio of the luminal to peritubular cell membrane . 3. In the rat SITS inhibits JNa moderately but the active H+ (glycodiazine) transport strongly. It does not affect the glucose transport. On the basis of these and other results a hypothesis of the interaction of Na+ and H+ (HCO3-) transport is given.

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Language(s): eng - English
 Dates: 1977
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-4684-3279-4_1
 Degree: -

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Title: Proceedings of the Ninth Annual Rochester International Conference on Environmental Toxicity
Place of Event: Rochester, New York
Start-/End Date: 1976-05-24 - 1976-05-26

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Title: Advances in Experimental Medicine and Biology
  Subtitle : Membrane Toxicity
Source Genre: Series
 Creator(s):
Miller, Morton W.1, Editor
Shamoo, Adil E.1, Author
Affiliations:
1 University of Rochester, Rochester, New York, USA, ou_persistent22            
Publ. Info: New York, London : Plenum Press
Pages: 553 Volume / Issue: 84 Sequence Number: - Start / End Page: 3 - 13 Identifier: DOI: 10.1007/978-1-4684-3279-4
ISBN: 978-1-4684-3279-4
n.a.: 978-1-4684-3281-7