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  A new-to-nature carboxylation module to improve natural and synthetic CO2 fixation

Scheffen, M., Marchal, D. G., Beneyton, T., Schuller, S. K., Klose, M., Diehl, C., et al. (2021). A new-to-nature carboxylation module to improve natural and synthetic CO2 fixation. Nature Catalysis, 4, 104-115. doi:10.1038/s41929-020-00557-y.

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 Creators:
Scheffen, Marieke1, Author
Marchal, Daniel G.1, Author
Beneyton, Thomas1, Author
Schuller, Sandra K.2, Author           
Klose, Melanie1, Author
Diehl, Christoph1, Author
Lehmann, Jessica1, Author
Pfister, Pascal1, Author
Carrillo, Martina1, Author
He, Hai1, Author
Aslan, Selcuk1, Author
Cortina, Nina S.1, Author
Claus, Peter1, Author
Bollschweiler, Daniel3, Author           
Baret, Jean-Christophe1, Author
Schuller, Jan M.2, Author           
Zarzycki, Jan1, Author
Bar-Even, Arren1, Author
Erb, Tobias J.1, Author
Affiliations:
1external, ou_persistent22              
2Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              
3Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: CRYSTAL-STRUCTURE; PHOTORESPIRATORY BYPASS; SUBSTRATE-BINDING; ETHYLENE-GLYCOL; BETA-SUBUNIT; ENZYME; METABOLISM; EVOLUTION; PATHWAY; CYCLEChemistry;
 Abstract: The capture of CO2 by carboxylases is key to sustainable biocatalysis and a carbon-neutral bio-economy, yet currently limited to few naturally existing enzymes. Here, we developed glycolyl-CoA carboxylase (GCC), a new-to-nature enzyme, by combining rational design, high-throughput microfluidics and microplate screens. During this process, GCC's catalytic efficiency improved by three orders of magnitude to match the properties of natural CO2-fixing enzymes. We verified our active-site redesign with an atomic-resolution, 1.96-angstrom cryo-electron microscopy structure and engineered two more enzymes that, together with GCC, form a carboxylation module for the conversion of glycolate (C-2) to glycerate (C-3). We demonstrate how this module can be interfaced with natural photorespiration, ethylene glycol conversion and synthetic CO2 fixation. Based on stoichiometrical calculations, GCC is predicted to increase the carbon efficiency of all of these processes by up to 150% while reducing their theoretical energy demand, showcasing how expanding the solution space of natural metabolism provides new opportunities for biotechnology and agriculture.

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Language(s): eng - English
 Dates: 2021-01
 Publication Status: Published online
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: Nature Catalysis
  Abbreviation : Nat. Catal.
Source Genre: Journal
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Publ. Info: New York : Nature Publishing Group
Pages: - Volume / Issue: 4 Sequence Number: - Start / End Page: 104 - 115 Identifier: ISSN: 25201158
CoNE: https://pure.mpg.de/cone/journals/resource/25201158