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  Fine‐Tuning Protein Self‐Organization by Orthogonal Chemo‐Optogenetic Tools

Sun, H., Jia, H., Ramirez-Diaz, D. A., Budisa, N., & Schwille, P. (2021). Fine‐Tuning Protein Self‐Organization by Orthogonal Chemo‐Optogenetic Tools. Angewandte Chemie International Edition, 60(9), 1433-7851. doi:10.1002/anie.202008691.

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© 2020 The Authors. Open access funding enabled and organized by Projekt DEAL.
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 Creators:
Sun, Huan1, Author
Jia, Haiyang2, Author              
Ramirez-Diaz, Diego A.2, Author              
Budisa, Nediljko1, Author
Schwille, Petra2, Author              
Affiliations:
1external, ou_persistent22              
2Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: OPTICAL CONTROL; GENETIC-CODE; FTSZ; CELL; RECONSTITUTIONChemistry; bottom-up reconstitution; chemo-optogenetic tools; FtsZ; genetic code expansion; membranes; synthetic biology;
 Abstract: A universal gain-of-function approach for the spatiotemporal control of protein activity is highly desirable when reconstituting biological modules in vitro. Here we used orthogonal translation with a photocaged amino acid to map and elucidate molecular mechanisms in the self-organization of the prokaryotic filamentous cell-division protein (FtsZ) that is highly relevant for the assembly of the division ring in bacteria. We masked a tyrosine residue of FtsZ by site-specific incorporation of a photocaged tyrosine analogue. While the mutant still shows self-assembly into filaments, dynamic self-organization into ring patterns can no longer be observed. UV-mediated uncaging revealed that tyrosine 222 is essential for the regulation of the protein's GTPase activity, self-organization, and treadmilling dynamics. Thus, the light-mediated assembly of functional protein modules appears to be a promising minimal-regulation strategy for building up molecular complexity towards a minimal cell.

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Language(s): eng - English
 Dates: 2021-01
 Publication Status: Published online
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000605644300001
DOI: 10.1002/anie.202008691
 Degree: -

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Project name : Molecular Principles of Synthetic Biology
Grant ID : GRK2062
Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft

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Title: Angewandte Chemie International Edition
  Abbreviation : Angew. Chem., Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 60 (9) Sequence Number: - Start / End Page: 1433 - 7851 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851