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  Structure of a transcribing RNA polymerase II–U1 snRNP complex

Zhang, S., Aibara, S., Vos, S. M., Agafonov, D., Lührmann, R., & Cramer, P. (2021). Structure of a transcribing RNA polymerase II–U1 snRNP complex. Science, 371(6526), 305-309. doi:10.1126/science.abf1870.

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 Creators:
Zhang, S.1, Author              
Aibara, S.1, Author              
Vos, S. M.1, Author              
Agafonov, D.2, Author              
Lührmann, R.2, Author              
Cramer, P.1, Author              
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1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              
2Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578576              

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 Abstract: To initiate cotranscriptional splicing, RNA polymerase II (Pol II) recruits the U1 small nuclear ribonucleoprotein particle (U1 snRNP) to nascent precursor messenger RNA (pre-mRNA). Here, we report the cryo–electron microscopy structure of a mammalian transcribing Pol II–U1 snRNP complex. The structure reveals that Pol II and U1 snRNP interact directly. This interaction positions the pre-mRNA 5′ splice site near the RNA exit site of Pol II. Extension of pre-mRNA retains the 5′ splice site, leading to the formation of a “growing intron loop.” Loop formation may facilitate scanning of nascent pre-mRNA for the 3′ splice site, functional pairing of distant intron ends, and prespliceosome assembly. Our results provide a starting point for a mechanistic analysis of cotranscriptional spliceosome assembly and the biogenesis of mRNA isoforms by alternative splicing.

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Language(s): eng - English
 Dates: 2021-01-152021-01-15
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1126/science.abf1870
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Title: Science
Source Genre: Journal
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Pages: - Volume / Issue: 371 (6526) Sequence Number: - Start / End Page: 305 - 309 Identifier: -