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  Solubilization of ligand-stabilized vasopressin receptors from plasma membranes of bovine kidney and rat liver

Boer, R., Crause, P., & Fahrenholz, F. (1983). Solubilization of ligand-stabilized vasopressin receptors from plasma membranes of bovine kidney and rat liver. Biochemical and Biophysical Research Communications, 116(1), 91-98. doi:10.1016/0006-291X(83)90385-6.

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 Creators:
Boer, Rainer1, Author              
Crause, Peter1, Author              
Fahrenholz, Falk1, Author              
Affiliations:
1Department of Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_3264819              

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 Abstract: The solubilization of vasopressin receptors from plasma membranes of bovine kidney and rat liver by different detergents was investigated. A prerequisite for the extraction of vasopressin receptors retaining binding affinity for their ligand was the stabilization of the receptors by the prior formation of the membrane-bound hormone-receptor complexes. The vasopressin-receptor complexes from both kidney and liver membranes were solubilized in a high yield with dodecyl-β-D-maltoside and 3-laurylamido-N,N′-dimethylpropylaminoxide. Several other nonionic detergents including octyl-β-D-glucopyranoside effectively extracted the hepatic vasopressin receptor. For the hormone-receptor complex solubilized from bovine kidney with dodecyl-β-D-maltoside, a Stokes' radius of 5.8 nm was determined.

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Language(s): eng - English
 Dates: 1983-07-042004-12-081983-10-14
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0006-291X(83)90385-6
 Degree: -

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Title: Biochemical and Biophysical Research Communications
  Other : Biochem. Biophys. Res. Commun.
Source Genre: Journal
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Publ. Info: Orlando, Fla. : Academic Press
Pages: - Volume / Issue: 116 (1) Sequence Number: - Start / End Page: 91 - 98 Identifier: ISSN: 0006-291X
CoNE: https://pure.mpg.de/cone/journals/resource/954922652205_1