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  Far-red switching DNA probes for live cell nanoscopy

Bucevicius, J., Gilat, T., & Lukinavicius, G. (2020). Far-red switching DNA probes for live cell nanoscopy. Chemical Communications, 56(94), 14797-14800. doi:10.1039/D0CC06759H.

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Bucevicius, J.1, Author              
Gilat, T.2, Author              
Lukinavicius, G.1, Author              
Affiliations:
1Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              
2Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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 Abstract: Herein we present DNA probes composed of Hoechst 33258 and spontaneously blinking far-red hydroxymethyl silicon-rhodamine (HMSiR). The best performing probe, 5-HMSiR-Hoechst, contains the 5′-regioisomer, shows ∼400-fold fluorescence increase upon DNA binding and is compatible with wash-free single molecule localization and 3D stimulated emission depletion microscopy of chromatin nanostructures in living cells.

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Language(s): eng - English
 Dates: 2020-10-272020-12-07
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1039/D0CC06759H
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Title: Chemical Communications
Source Genre: Journal
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Pages: - Volume / Issue: 56 (94) Sequence Number: - Start / End Page: 14797 - 14800 Identifier: -