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  Defining the architecture of the human TIM22 complex by chemical crosslinking

Valpadashi, A., Callegari, S., Linden, A., Neumann, P., Ficner, R., Urlaub, H., et al. (2021). Defining the architecture of the human TIM22 complex by chemical crosslinking. FEBS Letters, 595(2), 157-168. doi:10.1002/1873-3468.13978.

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 Creators:
Valpadashi, A., Author
Callegari, S., Author
Linden, A.1, Author              
Neumann, P., Author
Ficner, R., Author
Urlaub, H.1, Author              
Deckers, M., Author
Rehling, P.2, Author              
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              
2Max Planck Fellow Peter Rehling, ou_1298545              

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Free keywords: carrier translocase, crosslinking‐mass spectrometry, mitochondria, protein translocation, TIM22
 Abstract: The majority of mitochondrial proteins are nuclear encoded and imported into mitochondria as precursor proteins via dedicated translocases. The translocase of the inner membrane 22 (TIM22) is a multisubunit molecular machine specialized for the translocation of hydrophobic, multi‐transmembrane‐spanning proteins with internal targeting signals into the inner mitochondrial membrane. Here, we undertook a crosslinking‐mass spectrometry (XL‐MS) approach to determine the molecular arrangement of subunits of the human TIM22 complex. Crosslinking of the isolated TIM22 complex using the BS3 crosslinker resulted in the broad generation of crosslinks across the majority of TIM22 components, including the small TIM chaperone complex. The crosslinking data uncovered several unexpected features, opening new avenues for a deeper investigation into the steps required for TIM22‐mediated translocation in humans.

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Language(s): eng - English
 Dates: 2020-10-302021-01-24
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1002/1873-3468.13978
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Title: FEBS Letters
Source Genre: Journal
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Pages: - Volume / Issue: 595 (2) Sequence Number: - Start / End Page: 157 - 168 Identifier: -