English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Lycopene β-cyclase expression influences plant physiology, development and metabolism in tobacco plants

Kossler, S., Armarego-Marriott, T., Tarkowska, D., Tureckova, V., Agrawal, S., Mi, J., et al. (2021). Lycopene β-cyclase expression influences plant physiology, development and metabolism in tobacco plants. Journal of Experimental Botany, 72(7), 2544-2569. doi:10.1093/jxb/erab029.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files

Locators

show
hide
Locator:
Link (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Kossler , Stella1, Author
Armarego-Marriott, T.2, Author              
Tarkowska, Danuse1, Author
Tureckova, Veronika1, Author
Agrawal, S.2, Author              
Mi, Jianing1, Author
Perez da Sousa, L.3, Author              
Schöttler, M. A.4, Author              
Schadach, A.2, Author              
Fröhlich, A.5, Author              
Bock, R.2, Author              
Al-Babili, Salim1, Author
Ruf, S.2, Author              
Sampathkumar, A.5, Author              
Moreno, J.C.2, Author              
Affiliations:
1External Organizations, ou_persistent22              
2Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              
3Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
4Photosynthesis Research, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753323              
5Plant Cell Biology and Microscopy, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2253647              

Content

show
hide
Free keywords: -
 Abstract: Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. β-carotene is generated from lycopene by the lycopene β-cyclase (LCYB). Previously, we demonstrated that the introduction of the Daucus carota (carrot) DcLCYB1 gene into tobacco (cultivar Xanthi) resulted in increased levels of abscisic acid (ABA) and especially gibberellins (GAs), resulting in increased plant yield. In order to understand this phenomenon prior exporting this genetic strategy to crops, we generated tobacco (cultivar Petit Havana) mutants that exhibited a wide range of LCYB expression. Transplastomic plants expressing DcLCYB1 at high levels showed a wild-type-like growth, even though their pigment content was increased, and their leaf GA content was reduced. RNAi NtLCYB lines showed different reductions in NtLCYB transcript abundance, correlating with reduced pigment content and plant variegation. Photosynthesis (leaf absorptance, Fv/Fm, and ETRII) and plant growth were impaired. Remarkably, drastic changes in phytohormone content also occurred in the RNAi lines. However, external application of phytohormones was not sufficient to rescue their phenotypes, suggesting that altered photosynthetic efficiency might be another important factor explaining their reduced biomass. These results show that LCYB expression influences plant biomass by different mechanisms and suggests thresholds for LCYB expression levels that might be beneficial/detrimental for plant growth.

Details

show
hide
Language(s): eng - English
 Dates: 2021
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1093/jxb/erab029
BibTex Citekey: PMID:33484250
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Experimental Botany
  Other : J. Exp. Bot
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford : Oxford University Press [etc.]
Pages: - Volume / Issue: 72 (7) Sequence Number: - Start / End Page: 2544 - 2569 Identifier: ISSN: 0022-0957
CoNE: https://pure.mpg.de/cone/journals/resource/954925413883