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  Knock-down of the plastid-encoded acetyl-CoA carboxylase gene uncovers functions in metabolism and development

Caroca, R., Howell, K. A., Malinova, I., Burgos, A., Tiller, N., Pellizzer, T., et al. (2021). Knock-down of the plastid-encoded acetyl-CoA carboxylase gene uncovers functions in metabolism and development. Plant Physiology, 185(3), 1091-1110. doi:10.1093/plphys/kiaa106.

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Caroca, R.1, Author              
Howell, K. A.1, Author              
Malinova, I.2, Author              
Burgos, A.3, Author              
Tiller, N.1, Author              
Pellizzer, T.2, Author              
Annunziata, Maria Grazia4, Author              
Hasse, C1, Author              
Ruf, S.1, Author              
Karcher, D.1, Author              
Bock, R.1, Author              
Affiliations:
1Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              
2Cytoplasmic and Evolutionary Genetics, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753324              
3Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753340              
4System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              

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 Abstract: De novo fatty acid biosynthesis in plants relies on a prokaryotic-type acetyl-CoA carboxylase (ACCase) that resides in the plastid compartment. Whereas three subunits are encoded by nuclear genes, the plastid gene (accD) encodes the β-carboxyltransferase subunit of ACCase and is essential for cell viability. To facilitate the functional analysis of accD, we pursued a transplastomic knock-down strategy in tobacco (Nicotiana tabacum). By introducing point mutations into the translational start codon of accD, we obtained stable transplastomic lines with altered ACCase activity. Replacement of the standard initiator codon AUG with UUG strongly reduced AccD expression, whereas replacement with GUG had no detectable effects. AccD knock-down mutants displayed reduced ACCase activity, which resulted in changes in the levels of many but not all species of cellular lipids. Limiting fatty acid availability caused a wide range of macroscopic, microscopic and biochemical phenotypes, including impaired chloroplast division, reduced seed set, and altered storage metabolism. Finally, while the mutants displayed reduced growth under photoautotrophic conditions, they showed exaggerated growth under heterotrophic conditions, thus uncovering an unexpected antagonistic role of AccD activity in autotrophic and heterotrophic growth.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: DOI: 10.1093/plphys/kiaa106
BibTex Citekey: 10.1093/plphys/kiaa106
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 185 (3) Sequence Number: - Start / End Page: 1091 - 1110 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438