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  Laser Targeted Oligo Ligation (LTOL) to Identify DNA Sequences in the Vicinity of a Single Subnuclear Structure in a Single Cell

Anchel, D., & Ching, R. (2020). Laser Targeted Oligo Ligation (LTOL) to Identify DNA Sequences in the Vicinity of a Single Subnuclear Structure in a Single Cell. Methods in Molecular Biology, 2175, 11-21. doi:org/10.1007/978-1-0716-0763-3_2,.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0007-DF48-E Version Permalink: https://hdl.handle.net/21.11116/0000-0008-718B-C
Genre: Journal Article

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Anchel-Ching 2020.pdf (Publisher version), 300KB
 
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Restricted (Max Planck Institute of Immunobiology and Epigenetics, MFIB; )
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2020
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Springer+Business Media, LLC, part of Springer Nature 2020
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https://link.springer.com/protocol/10.1007/978-1-0716-0763-3_2 (Publisher version)
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 Creators:
Anchel, David1, Author
Ching, Reagen2, Author           
Affiliations:
1External Organizations, ou_persistent22              
2Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243640              

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Free keywords: Nuclear organization, Subnuclear structures, Gene hubs, Single cell, Microscopy, Sequencing
 Abstract: Gene loci are organized around nuclear substructures, forming gene hubs which provide a level of transcriptional control. To date, most techniques used to investigate the genes in these hubs have been based on using material from bulk cells. This makes identifying specific gene associations difficult. Here we describe the Laser Targeted Oligo Ligation (LTOL) technique that was developed to identify DNA sequences around a single subnuclear structure on a single-cell basis by targeting these regions with two-photon irradiation.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: org/10.1007/978-1-0716-0763-3_2,
 Degree: -

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Title: Methods in Molecular Biology
  Other : Methods Mol. Biol.
Source Genre: Journal
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Publ. Info: US : Springer
Pages: - Volume / Issue: 2175 Sequence Number: - Start / End Page: 11 - 21 Identifier: ISSN: 1064-3745
CoNE: https://pure.mpg.de/cone/journals/resource/954927725544