hide
Free keywords:
Membrane protein; Chlorophyll-protein complex; Protein phosphorylation; Chromatography, column; Light harvesting complex
Abstract:
Ion-exchange column chromatography of 32P-labelled light-harvesting chlorophyll a/b-protein complex (LHC II) isolated from spinach or pea chloroplasts yielded in each case several fractions, differing with respect to polypeptide composition and 32P incorporation. Separation of native, phosphorylated and unphosphorylated complex on a preparative scale was achieved for spinach LHC II.