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  A laser temperature jump apparatus based on commercial parts equipped with highly sensitive spectrophotometric detection

Bremer, C., Bergbauer, R., Ruf, H., Bannister, J. J., & Grell, E. (1993). A laser temperature jump apparatus based on commercial parts equipped with highly sensitive spectrophotometric detection. Measurement Science and Technology, 4(12), 1385-1393. doi:10.1088/0957-0233/4/12/013.

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 Creators:
Bremer, Christoph1, Author           
Bergbauer, Rolf2, Author           
Ruf, Horst1, Author           
Bannister, John J.3, Author
Grell, Ernst1, Author           
Affiliations:
1Molecular Biophysics Group, Max Planck Institute of Biophysics, Max Planck Society, ou_3264820              
2Max Planck Institute of Biophysics, Max Planck Society, ou_2068288              
3Medioption Ltd., Richmond House, Manchester M1 3HZ, UK, ou_persistent22              

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 Abstract: A temperature jump apparatus is described which is based mainly on commercially available components. Temperature jumps of about 2 degrees C in about 150 µl of aqueous solutions can be produced within 10 µs by absorption of the infrared radiation of a pulsed atomic iodine laser. The time range available for kinetic experiments is about 30 µs to 1s. The necessary modifications of the commercial iodine laser are discussed. A prominent feature of the apparatus is the very high sensitivity of the spectrophotometric detection system in the visible spectral range. Signal-to-noise ratios of up to 2x104 are reached at a time resolution of 1 µs. A method is described to determine the size of the temperature jump of each individual experiment. Thus not only the relaxation times, which are independent of the size of small temperature jumps, can be evaluated but also the corresponding relaxation amplitudes can be determined in a precise manner. Furthermore, an effective method to recycle the laser medium i-C3F7I is described.

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Language(s): eng - English
 Dates: 1993-06-101993-08-171993-12-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1088/0957-0233/4/12/013
 Degree: -

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Title: Measurement Science and Technology
Source Genre: Journal
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Publ. Info: Bristol : IOP Pub.
Pages: - Volume / Issue: 4 (12) Sequence Number: - Start / End Page: 1385 - 1393 Identifier: ISSN: 0957-0233
CoNE: https://pure.mpg.de/cone/journals/resource/954927547263