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  Determination of kinetic parameters from chemical relaxation data discrimination between coupled two-step binding reactions differing in stoichiometry

Bremer, C., & Grell, E. (1994). Determination of kinetic parameters from chemical relaxation data discrimination between coupled two-step binding reactions differing in stoichiometry. European Biophysics Journal, 23, 217-226. doi:10.1007/BF01007613.

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 Creators:
Bremer, Christoph1, Author           
Grell, Ernst1, Author           
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              

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Free keywords: Chemical relaxation; Relaxation kinetics; Two-step binding reactions; Relaxation times; Kinetic parameters
 Abstract: The chemical relaxation times of two different two-step equilibrium reactions, characterized by a 1:1 binding process followed by a subsequent rearrangement step and a stepwise 1:2 binding reaction, are analyzed for the purpose of qualitative model discrimination and quantitative determination of kinetic parameters. The equations describing the dependences of the two reciprocal relaxation times on suitable concentrations are given for both models in the general case as well as for four different limiting situations which are characterized by well separated relaxation times. The conditions corresponding to the limiting cases are expressed in terms of strong, weak and no coupling between the two partial equilibrium steps involved in both models. The coupling strength depends on the rate constants as well as on the total concentrations of the reactants. Criteria to discriminate between these two reaction models under defined limiting conditions are developed. In the general case, the product of both reciprocal relaxation times can be used to distinguish both models. If only one relaxation time can be resolved experimentally, it is possible under conditions described to determine only a reduced set of individual rate constants for most of the limiting cases considered. If both relaxation times are observed, all rate constants are determinable in the general case as well as in most of the limiting cases discussed.

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Language(s): eng - English
 Dates: 1993-12-241994-05-171994-05-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/BF01007613
 Degree: -

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Title: European Biophysics Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Berlin : Springer
Pages: - Volume / Issue: 23 Sequence Number: - Start / End Page: 217 - 226 Identifier: ISSN: 0175-7571
CoNE: https://pure.mpg.de/cone/journals/resource/954925487773_1