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  Reassignment of cation-induced population of main conformational states of FITC-Na+/K+-ATPase as detected by fluorescence spectroscopy and characterized by equilibrium binding studies

Grell, E., Lewitzki, E., Ruf, H., & Doludda, M. (1994). Reassignment of cation-induced population of main conformational states of FITC-Na+/K+-ATPase as detected by fluorescence spectroscopy and characterized by equilibrium binding studies. In E. Bamberg (Ed.), The Sodium Pump (pp. 617-620). Darmstadt, Germany: Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt 1994.

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 Creators:
Grell, Ernst1, Author           
Lewitzki, Erwin1, Author           
Ruf, Horst1, Author           
Doludda, Michael1, Author           
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              

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Free keywords: Choline Chloride; Adenosine Triphosphatase; Cation Binding; Fluorescence Emission Intensity; High affInity Binding Site
 Abstract: To link proposed features of the Na+/K+-ATPase reaction cycle to the molecular structure, spectroscopic studies mainly concerning the elucidation of partial reactions are carried out. This can be done by introducing fluorescence labels such as the F1TC-group (1) which enables the study of alkali ion binding as a key feature of this ion pump. According to earlier studies, a correlation between the fluorescence emission intensity and the position of the E1/E2 equilibrium is suggested: low fluorescence intensity in the presence of K+ has been attributed to E2, high intensity to E1(1–5). Evidence will be presented that this correlation concerning E1 is not fulfilled. Membrane-bound Na+/K+-ATPase has been prepared from pig kidney according to (6), the FITC-enzyme according to (1); details are given in (7).

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Language(s): eng - English
 Dates: 1994
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-3-642-72511-1_110
 Degree: -

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Title: The Sodium Pump
  Subtitle : Structure Mechanism, Hormonal Control and its Role in Disease
Source Genre: Book
 Creator(s):
Bamberg, Ernst1, Editor           
Schoner, Wilhelm2, Author
Affiliations:
1 Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289            
2 Institut für Biochemie, Universität Gießen, Gießen, Germany, ou_persistent22            
Publ. Info: Darmstadt, Germany : Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt 1994
Pages: 906 Volume / Issue: - Sequence Number: - Start / End Page: 617 - 620 Identifier: DOI: 10.1007/978-3-642-72511-1
ISBN: 978-3-642-72513-5
ISBN: 978-3-642-72511-1