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  lin-Benzo-ATP and-ADP: Versatile fluorescent probes for spectroscopic and biochemical studies

Grell, E., Lewitzki, E., Bremer, C., Kramer-Schmitt, S., Weber, J., & Senior, A. E. (1994). lin-Benzo-ATP and-ADP: Versatile fluorescent probes for spectroscopic and biochemical studies. Journal of Fluorescence, 4(3), 247-250. doi:10.1007/BF01878458.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0007-FCBF-7 Version Permalink: https://hdl.handle.net/21.11116/0000-0007-FCC0-4
Genre: Journal Article

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 Creators:
Grell, Ernst1, Author           
Lewitzki, Erwin1, Author           
Bremer, Christoph1, Author           
Kramer-Schmitt, Sabine1, Author           
Weber, Joachim2, Author
Senior, Alan E. 2, Author
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2Department of Biochemistry, University of Rochester Medical Center, 14642, Rochester, New York, ou_persistent22              

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Free keywords: lin-Benzo-ATP; lin-benzo-ADP; dissociation constants; Mg2+ and Ca2+ binding; fluorescence spectra
 Abstract: lin-Benzo-adenine nucleotides can act not only as probes for fluorescence studies but also as structural active site probes for enzymes. To understand the basic properties oflin-benzo-ATP and-ADP, protolysis and Mg2+ and Ca2+, binding are investigated between pH 6.2 and pH 8.5 by spectrophotometric and spectrofluorometric titrations. Based on a reaction model, a set of equilibrium constants is determined which is consistent with all available experimental results. The pK values of the Mg2+ and Ca2+ complex oflin-benzo-ATP in the chosen medium are 4.6 and 4.1, respectively, and those for the corresponding diphosphate are 3.1 and 2.8, respectively. Fluorescence and absorption spectra are reported.

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Language(s): eng - English
 Dates: 1994-06-101993-10-181994-06-101994-09-01
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/BF01878458
PMID: 24233455
 Degree: -

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Title: Journal of Fluorescence
Source Genre: Journal
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Publ. Info: New York, NY : Kluwer Academic/Plenum Publishers
Pages: - Volume / Issue: 4 (3) Sequence Number: - Start / End Page: 247 - 250 Identifier: ISSN: 1053-0509
CoNE: https://pure.mpg.de/cone/journals/resource/954925594506