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キーワード:
brown rice, catechins, cytotoxicity assay, flavonoids, glycemic index, human nutrition, metabolite genome-wide association study (mGWAS), pericarp colour
要旨:
Summary Brown rice possesses various nutritionally dense bioactive phytochemicals exhibiting wide range of antioxidant, anticancer and antidiabetic properties known to promote various human health benefits. However, despite the wide claims made about the importance of brown rice for human nutrition the underlying metabolic diversity not systematically explored. Non-targeted metabolite profiling of developing and mature seeds of a diverse genetic panel of 320 rice cultivars allowed quantification of 117 metabolites. The metabolite genome-wide association study (mGWAS) detected genetic variants influencing diverse metabolic targets in developing and mature seeds. We further interlinked genetic variants on chromosome 7 (6.06-6.43 Mb region) with complex epistatic genetic interactions impacting multi-dimensional nutritional targets, including complex carbohydrate starch quality, glycemic index, antioxidant catechin and rice grain colour. Through this nutrigenomics approach rare gene bank accessions possessing genetic variants in bHLH and IPT5 genes were identified through haplotype enrichment. These variants were associated with low glycemic index, higher catechin, elevated total flavonoid contents and heightened antioxidant activity in the whole grain with elevated anti-cancer properties being confirmed on cancer cell lines. This multi-disciplinary nutrigenomics approach thus allowed us to discover the genetic basis of human-health conferring diversity in the metabolome of brown rice.
