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  Subcellular sequencing of single neurons reveals the dendritic transcriptome of GABAergic interneurons

Perez, J. D., Tom Dieck, S., Alvarez-Castelao, B., Tushev, G., Chan, I. C., & Schuman, E. M. (2021). Subcellular sequencing of single neurons reveals the dendritic transcriptome of GABAergic interneurons. eLife, 10: e63092. doi:10.7554/eLife.63092.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0007-EEDF-3 Version Permalink: https://hdl.handle.net/21.11116/0000-000A-5154-C
Genre: Journal Article

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elife-63092-v2.pdf (Publisher version), 6MB
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https://hdl.handle.net/21.11116/0000-000A-5155-B
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2021
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© 2021, Perez et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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https://creativecommons.org/licenses/by/4.0/

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https://www.ncbi.nlm.nih.gov/pubmed/33404500 (Any fulltext)
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 Creators:
Perez, J. D., Author
Tom Dieck, S., Author
Alvarez-Castelao, B., Author
Tushev, G., Author
Chan, I. C., Author
Schuman, Erin M.1, Author           
Affiliations:
1Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society, ou_2461710              

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Free keywords: neuroscience rat
 Abstract: Although mRNAs are localized in the processes of excitatory neurons, it is still unclear whether interneurons also localize a large population of mRNAs. In addition, the variability in the localized mRNA population within and between cell-types is unknown. Here we describe the unbiased transcriptomic characterization of the subcellular compartments of hundreds of single neurons. We separately profiled the dendritic and somatic transcriptomes of individual rat hippocampal neurons and investigated mRNA abundances in the soma and dendrites of single glutamatergic and GABAergic neurons. We found that, like their excitatory counterparts, interneurons contain a rich repertoire of ~4000 mRNAs. We observed more cell type-specific features among somatic transcriptomes than their associated dendritic transcriptomes. Finally, using cell-type specific metabolic labelling of isolated neurites, we demonstrated that the processes of Glutamatergic and, notably, GABAergic neurons were capable of local translation, suggesting mRNA localization and local translation is a general property of neurons.

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Language(s): eng - English
 Dates: 2020-10-152021-01-052021-01-21
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: PMID: 33404500
DOI: 10.7554/eLife.63092
ISSN: 2050-084X (Electronic)2050-084X (Linking)
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Project name : ERC-AdG NeuroRibo
Grant ID : 743216
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)
Project name : SFB 1080: Molecular and Cellular Mechanisms of Neural Homeostasis (Subproject B01)
Grant ID : 221828878
Funding program : Sonderforschungsbereiche (SFB)
Funding organization : Deutsche Forschungsgemeinschaft (DFG)
Project name : SFB 902: Molecular Principles of RNA-based Regulation
Grant ID : 161793742
Funding program : Sonderforschungsbereiche (SFB)
Funding organization : Deutsche Forschungsgemeinschaft (DFG)

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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 10 Sequence Number: e63092 Start / End Page: - Identifier: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X