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  Spatially Stable Mitochondrial Compartments Fuel Local Translation during Plasticity

Rangaraju, V., Lauterbach, M., & Schuman, E. M. (2019). Spatially Stable Mitochondrial Compartments Fuel Local Translation during Plasticity. Cell, 176(1-2), 73-84.e15. doi:10.1016/j.cell.2018.12.013.

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1-s2.0-S0092867418316271-main.pdf (Publisher version), 6MB
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2018
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 Creators:
Rangaraju, V., Author
Lauterbach, M., Author
Schuman, Erin M.1, Author              
Affiliations:
1Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society, ou_2461710              

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Free keywords: Animals Cytoskeleton/metabolism Dendrites/metabolism Dendritic Spines/metabolism Female Male Mitochondria/*metabolism/physiology Neuronal Plasticity/physiology Neurons/*metabolism Protein Biosynthesis/*physiology RNA, Messenger/metabolism Rats Rats, Sprague-Dawley Synapses/metabolism *atp *compartments *cytoskeleton *energy *local translation *mitochondria *nascent protein *protein synthesis *synaptic plasticity
 Abstract: Local translation meets protein turnover and plasticity demands at synapses, however, the location of its energy supply is unknown. We found that local translation in neurons is powered by mitochondria and not by glycolysis. Super-resolution microscopy revealed that dendritic mitochondria exist as stable compartments of single or multiple filaments. To test if these mitochondrial compartments can serve as local energy supply for synaptic translation, we stimulated individual synapses to induce morphological plasticity and visualized newly synthesized proteins. Depletion of local mitochondrial compartments abolished both the plasticity and the stimulus-induced synaptic translation. These mitochondrial compartments serve as spatially confined energy reserves, as local depletion of a mitochondrial compartment did not affect synaptic translation at remote spines. The length and stability of dendritic mitochondrial compartments and the spatial functional domain were altered by cytoskeletal disruption. These results indicate that cytoskeletally tethered local energy compartments exist in dendrites to fuel local translation during synaptic plasticity.

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Language(s): eng - English
 Dates: 2018-05-112018-12-072019-01-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: 30612742
DOI: 10.1016/j.cell.2018.12.013
ISSN: 1097-4172 (Electronic)0092-8674 (Linking)
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Project name : ERC-AdG NeuroRibo
Grant ID : 743216
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)
Project name : MSCA NEUROMITO
Grant ID : 657702
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)
Project name : SFB 1080: Molecular and Cellular Mechanisms of Neural Homeostasis (Subproject B01)
Grant ID : 221828878
Funding program : Sonderforschungsbereiche (SFB)
Funding organization : Deutsche Forschungsgemeinschaft (DFG)
Project name : SFB 902: Molecular Principles of RNA-based Regulation
Grant ID : 161793742
Funding program : Sonderforschungsbereiche (SFB)
Funding organization : Deutsche Forschungsgemeinschaft (DFG)

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Title: Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 176 (1-2) Sequence Number: - Start / End Page: 73 - 84.e15 Identifier: ISSN: 0092-8674
CoNE: https://pure.mpg.de/cone/journals/resource/954925463183