Direct visualization of newly synthesized target proteins in situ

tom Dieck, S.; Kochen, L.; Hanus, C.; Heumuller, M.; Bartnik, I.; Nassim-Assir, B.; Merk, K.; Mosler, T.; Garg, S.; Bunse, S.; Tirrell, D. A.; Schuman, Erin M.

doi:10.1038/nmeth.3319

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Direct visualization of newly synthesized target proteins in situ

tom Dieck, S., Kochen, L., Hanus, C., Heumuller, M., Bartnik, I., Nassim-Assir, B., et al. (2015). Direct visualization of newly synthesized target proteins in situ. Nat Methods, 12(5), 411-4. doi:10.1038/nmeth.3319.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0007-EF13-7 Version Permalink: https://hdl.handle.net/21.11116/0000-0007-EF14-6

Genre: Journal Article

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https://www.ncbi.nlm.nih.gov/pubmed/25775042 (Any fulltext) Open Access status unknown

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Creators:
tom Dieck, S., Author
Kochen, L., Author
Hanus, C., Author
Heumuller, M., Author
Bartnik, I., Author
Nassim-Assir, B., Author
Merk, K., Author
Mosler, T., Author
Garg, S., Author
Bunse, S., Author
Tirrell, D. A., Author
Schuman, Erin M.¹, Author

Affiliations:
1Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society, ou_2461710

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Free keywords: Animals Antibodies Cells, Cultured Fibroblasts/*metabolism Gene Expression Regulation/physiology Hippocampus/cytology Mice Neurons/metabolism Proteins/*chemistry/*metabolism Rats Staining and Labeling

Abstract: Protein synthesis is a dynamic process that tunes the cellular proteome in response to internal and external demands. Metabolic labeling approaches identify the general proteomic response but cannot visualize specific newly synthesized proteins within cells. Here we describe a technique that couples noncanonical amino acid tagging or puromycylation with the proximity ligation assay to visualize specific newly synthesized proteins and monitor their origin, redistribution and turnover in situ.

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Dates: Date issued: 2015-03-17

Publication Status: Issued

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Identifiers: Other: 25775042
DOI: 10.1038/nmeth.3319
ISSN: 1548-7105 (Electronic)1548-7091 (Linking)

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Title: Nat Methods

Source Genre: Journal

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Pages: - Volume / Issue: 12 (5) Sequence Number: - Start / End Page: 411 - 4 Identifier: -