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  Neuronal NT-3 is not required for synaptic transmission or long-term potentiation in area CA1 of the adult rat hippocampus

Ma, L., Reis, G., Parada, L. F., & Schuman, E. M. (1999). Neuronal NT-3 is not required for synaptic transmission or long-term potentiation in area CA1 of the adult rat hippocampus. Learn Mem, 6(3), 267-75.

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Ma, L., Author
Reis, G., Author
Parada, L. F., Author
Schuman, Erin M.1, Author              
Affiliations:
1Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society, ou_2461710              

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Free keywords: Animals Blotting, Northern Blotting, Southern Electrophysiology Gene Deletion Hippocampus/cytology/*physiology Image Processing, Computer-Assisted In Vitro Techniques Long-Term Potentiation/*physiology Mice Mice, Knockout Nerve Growth Factors/*genetics/*physiology Neurons/*physiology Neurotrophin 3 Rats Synaptic Transmission/*physiology
 Abstract: Neurotrophic factors, including BDNF and NT-3, have been implicated in the regulation of synaptic transmission and plasticity. Previous attempts to analyze synaptic transmission and plasticity in mice lacking the NT-3 gene have been hampered by the early death of the NT-3 homozygous knockout animals. We have bypassed this problem by examining synaptic transmission in mice in which the NT-3 gene is deleted in neurons later in development, by crossing animals expressing the CRE recombinase driven by the synapsin I promoter to animals in which the NT-3 gene is floxed. We conducted blind field potential recordings at the Schaffer collateral-CA1 synapse in hippocampal slices from homozygous knockout and wild-type mice. We examined the following indices of synaptic transmission: (1) input-output relationship; (2) paired-pulse facilitation; (3) post-tetanic potentiation; and (4) long-term potentiation: induced by two different protocols: (a) two trains of 100-Hz stimulation and (b) theta burst stimulation. We found no difference between the knockout and wild-type mice in any of the above measurements. These results suggest that neuronal NT-3 does not play an essential role in normal synaptic transmission and some forms of plasticity in the mouse hippocampus.

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 Dates: 1999-09-24
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: Other: 10492008
ISSN: 1072-0502 (Print)1072-0502 (Linking)
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Title: Learn Mem
Source Genre: Journal
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Pages: - Volume / Issue: 6 (3) Sequence Number: - Start / End Page: 267 - 75 Identifier: -