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  Plant Chromatin Immunoprecipitation

Villar, C. B., & Köhler, C. (2010). Plant Chromatin Immunoprecipitation. In L. Henning (Ed.), Plant Developmental Biology (pp. 401-411).

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 Creators:
Villar, Corina B.R.1, Author
Köhler, C.2, Author              
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1external, ou_persistent22              
2External Organizations, ou_persistent22              

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 Abstract: Development of multicellular organisms is based on specialized gene expression programs. Because chromatin establishes the environment for transcription, understanding composition and dynamics of chromatin is an important part of developmental biology. The knowledge about chromatin has been greatly advanced by the chromatin immunoprecipitation (ChIP) technique, because ChIP allows to map the position of proteins as well as modifications of DNA and histones to specific genomic regions. Although ChIP has been applied to a wide range of model organisms, including Arabidopsis, it remains a challenging technique, and a careful experimental setup including appropriate positive and negative controls are required to obtain reliable results. Here, we describe a ChIP protocol adapted for material from Arabidopsis, which we routinely apply in our laboratory, and we discuss required controls and methods for data analysis.

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Language(s): eng - English
 Dates: 2010
 Publication Status: Published in print
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Title: Plant Developmental Biology
Source Genre: Book
 Creator(s):
Henning, Lars1, Editor
Köhler, C.1, Author            
Affiliations:
1 External Organizations, ou_persistent22            
Publ. Info: -
Pages: - Volume / Issue: 655 Sequence Number: - Start / End Page: 401 - 411 Identifier: -

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Title: Methods in Molecular Biology
Source Genre: Series
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Publ. Info: -
Pages: - Volume / Issue: 655 Sequence Number: - Start / End Page: 401 - 411 Identifier: -