Spatiotemporal sequence of mesoderm and endoderm lineage segregation during 
mouse gastrulation

Probst, Simone; Sagar,; Tosic, Jelena; Schwan, Carsten; Grün, Dominic; Arnold, Sebastian J

doi:10.1242/dev.193789

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Spatiotemporal sequence of mesoderm and endoderm lineage segregation during mouse gastrulation

Probst, S., Sagar, Tosic, J., Schwan, C., Grün, D., & Arnold, S. J. (2021). Spatiotemporal sequence of mesoderm and endoderm lineage segregation during mouse gastrulation. Development, 148: dev193789. doi:10.1242/dev.193789.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0007-ED5D-7 Version Permalink: https://hdl.handle.net/21.11116/0000-000A-2CE5-3

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https://journals.biologists.com/dev/article/148/1/dev193789/223197/Spatiotemporal-sequence-of-mesoderm-and-endoderm (Publisher version) Open Access status unknown

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Creators:
Probst, Simone¹, Author
Sagar², Author
Tosic, Jelena¹, Author
Schwan, Carsten¹, Author
Grün, Dominic², Author
Arnold, Sebastian J¹, Author

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1External Organizations, ou_persistent22
2Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243642

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Free keywords: Definitive endoderm; Eomes; Gastrulation; Lineage specification; Mesoderm; Mouse embryo

Abstract: Anterior mesoderm (AM) and definitive endoderm (DE) progenitors represent the earliest embryonic cell types that are specified during germ layer formation at the primitive streak (PS) of the mouse embryo. Genetic experiments indicate that both lineages segregate from Eomes-expressing progenitors in response to different Nodal signaling levels. However, the precise spatiotemporal pattern of the emergence of these cell types and molecular details of lineage segregation remain unexplored. We combined genetic fate labeling and imaging approaches with single-cell RNA sequencing (scRNA-seq) to follow the transcriptional identities and define lineage trajectories of Eomes-dependent cell types. Accordingly, all cells moving through the PS during the first day of gastrulation express Eomes AM and DE specification occurs before cells leave the PS from Eomes-positive progenitors in a distinct spatiotemporal pattern. ScRNA-seq analysis further suggested the immediate and complete separation of AM and DE lineages from Eomes-expressing cells as last common bipotential progenitor.

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Language(s): eng - English

Dates: Published Online: 2021-01-07

Publication Status: Published online

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Rev. Type: Peer

Identifiers: DOI: 10.1242/dev.193789

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Title: Development

Other : Development

Source Genre: Journal

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Publ. Info: Cambridge, Cambridgeshire : Company of Biologists

Pages: - Volume / Issue: 148 Sequence Number: dev193789 Start / End Page: - Identifier: ISSN: 0950-1991
CoNE: https://pure.mpg.de/cone/journals/resource/954927546241