ausblenden:
Schlagwörter:
Oregon Green BAPTA-1
calcium imaging
electrophysiology
locust
olfaction
projection neuron
spiking activity
two-photon microscopy
Zusammenfassung:
Combining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500-750 Hz) and spike output in principal neurons in the locust antennal lobe. Our goal was to determine whether the firing rate of individual neurons can be estimated in vivo with calcium imaging and, if so, to measure directly the accuracy and resolution of our estimates. Using the calcium indicator Oregon Green BAPTA-1, we describe a simple method to reconstruct firing rates from dendritic calcium signals with 80-90% accuracy and 50 ms temporal resolution.
