hide
Free keywords:
Afferent Pathways
Animals
Female
Grasshoppers/*anatomy & histology
Horseradish Peroxidase
Immunohistochemistry/methods
Interneurons/*chemistry
Isoquinolines
Male
Microscopy, Electron
Microscopy, Fluorescence
Staining and Labeling
gamma-Aminobutyric Acid/*analysis/physiology
Abstract:
Intracellular labelling of locust intersegmental interneurones with lucifer yellow or horseradish peroxidase was carried out in combination with light and electron microscope immunocytochemistry by using an antibody raised against gamma amino butyric acid (GABA). Fifteen percent (four out of 27) of intracellularly stained interneurones showed GABA-like immunoreactivity. This is in agreement with previous physiological observations that 20% of the interneurones in this population make inhibitory output connections in the metathoracic ganglion. GABA-like immunoreactivity was also found in processes presynaptic to the interneurones in the mesothoracic ganglion. The presence of such immunoreactive inputs onto the intersegmental interneurones correlates well with physiological evidence that their receptive fields are in part shaped by direct input from GABA-ergic spiking local interneurones.
