Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
 Lokale TagsFreigabegeschichteDetailsÜbersicht
  ATP-driven proton transport in vesicles from the kidney cortex

Sabolić, I., & Burckhardt, G. (1990). ATP-driven proton transport in vesicles from the kidney cortex. In S. Fleischer, & B. Fleischer (Eds.), Methods in Enyzmology (pp. 505-520). Academic Press. doi:10.1016/0076-6879(90)91033-3.

Item is

 

einblenden: alle ausblenden: alle

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-0007-F124-0 Versions-Permalink: https://hdl.handle.net/21.11116/0000-0007-F130-2
Genre: Buchkapitel

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Sabolić, Ivan1, Autor           
Burckhardt, Gerhard1, Autor           
Affiliations:
1Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Publisher Summary:
This chapter describes an improved preparation of endocytotic vesicles from rat renal cortex. This method yields vesicles that are more enriched in proton pump activity. The preparation of endocytotic vesicles takes about 6 hr and does not require an ultracentrifuge. Therefore, endocytotic vesicles from kidney can serve as an excellent model to study the characteristics of the vacuolar proton pump (H+-ATPase). This chapter describes the way of tissue homogenization is of critical importance for the success of the preparation. Although the proton pump (H+- adenosine tri-phosphate (ATP)-ase) in isolated endocytotic vesicles is strongly inhibited by N,N'-dicyclohexylcarbodiimide (DCCD), N-Ethylmaleimide (NEM), and diethylstilbestrol (DES), neither of these inhibitors can be used as a specific marker for the pump. Experiments showing the distribution of inhibitor-sensitive ATPases indicate that much higher activities of DCCD-, NEM-, and DES-sensitive ATPases are present in fractions of the Percoll gradient that do not contain endocytotic vesicles with an ATP-driven proton pump.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2004-01-071990
 Publikationsstatus: Erschienen
 Seiten: 15
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1016/0076-6879(90)91033-3
PMID: 1963653
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Methods in Enyzmology
  Untertitel : Biomembranes Part V: Cellular and Subcellular Transport: Epithelial Cells
Genre der Quelle: Reihe
 Urheber:
Fleischer, Sidney, Herausgeber
Fleischer, Becca , Herausgeber
Affiliations:
-
Ort, Verlag, Ausgabe: Academic Press
Seiten: 939 Band / Heft: 191 Artikelnummer: - Start- / Endseite: 505 - 520 Identifikator: ISBN: 978-0-12-182092-3
ISSN: 0076-6879