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  Molecular and cellular dynamics of the 26S proteasome

Sakata, E., Eisele, M. R., & Baumeister, W. (2021). Molecular and cellular dynamics of the 26S proteasome. Biochimica et Biophysica Acta-Proteins and Proteomics, 1869(3): 140583. doi:10.1016/j.bbapap.2020.140583.

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1-s2.0-S1570963920302302-main.pdf (Publisher version), 9MB
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 Creators:
Sakata, Eri1, Author              
Eisele, Markus R.1, Author              
Baumeister, Wolfgang1, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: CRYO-EM STRUCTURE; 20S PROTEASOME; CRYOELECTRON TOMOGRAPHY; STRUCTURAL INSIGHTS; CONFORMATIONAL LANDSCAPE; UBIQUITIN RECEPTORS; REVEALS MECHANISMS; CRYSTAL-STRUCTURE; CHAIN-BINDING; REPEAT DOMAINBiochemistry & Molecular Biology; Biophysics; 26S proteasome; cryo-electron microscopy; cryo-electron tomography; structural dynamics; AAA plus ATPases; single particle analysis;
 Abstract: In eukaryotic cells, the ubiquitin-proteasome system serves to remove proteins that are either dysfunctional or no longer needed. The 26S proteasome is a 2.5 MDa multisubunit complex comprising the 20S core particle, where degradation is executed, and one or two regulatory particles which prepare substrates for degradation. Whereas the 20S core particles of several species had been studied extensively by X-ray crystallography, the 26S holocomplex structure had remained elusive for a long time. Recent advances in single-particle cryo-electron microscopy have changed the situation and provided atomic resolution models of this intriguing molecular machine and its dynamics. Besides, cryo-electron tomography enables structural studies in situ, providing molecular resolution images of macromolecules inside pristinely preserved cellular environments. This has greatly contributed to our understanding of proteasome dynamics in the context of cells.

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Language(s): eng - English
 Dates: 2021-03
 Publication Status: Published online
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Project name : EXC 2067/1- 390729940 and CRC889/Project A11
Grant ID : -
Funding program : Germany’s Excellence Strategy
Funding organization : Deutsche Forschungsgemeinschaft
Project name : SFB-1035/Project A01 to W.B. and E.S.
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Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft
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Grant ID : PCIG14-GA-2013-631577
Funding program : Marie Curie Career Integration grant
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Title: Biochimica et Biophysica Acta-Proteins and Proteomics
  Other : BBA-Proteins Proteomics
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 1869 (3) Sequence Number: 140583 Start / End Page: - Identifier: ISSN: 1570-9639
CoNE: https://pure.mpg.de/cone/journals/resource/954926938702_5