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  Mutations Affecting HVO_1357 or HVO_2248 Cause Hypermotility in Haloferax volcanii, Suggesting Roles in Motility Regulation

Collins, M., Afolayan, S., Igiraneza, A. B., Schiller, H., Krespan, E., Beiting, D. P., et al. (2021). Mutations Affecting HVO_1357 or HVO_2248 Cause Hypermotility in Haloferax volcanii, Suggesting Roles in Motility Regulation. Genes, 12(1): 58. doi:10.3390/genes12010058.

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 Creators:
Collins, Michiyah1, Author
Afolayan, Simisola1, Author
Igiraneza, Aime B.1, Author
Schiller, Heather1, Author
Krespan, Elise1, Author
Beiting, Daniel P.1, Author
Dyall-Smith, Mike2, Author              
Pfeiffer, Friedhelm2, Author              
Pohlschroder, Mechthild1, Author
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1external, ou_persistent22              
2Habermann, Bianca / Computational Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1832284              

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Free keywords: Genetics & Heredity; Haloferax volcanii; archaea; swimming motility; archaella; chemotaxis; hypermotility selection; transposon mutagenesis; two-component regulatory system; extremophiles;
 Abstract: Motility regulation plays a key role in prokaryotic responses to environmental stimuli. Here, we used a motility screen and selection to isolate hypermotile Haloferax volcanii mutants from a transposon insertion library. Whole genome sequencing revealed that hypermotile mutants were predominantly affected in two genes that encode HVO_1357 and HVO_2248. Alterations of these genes comprised not only transposon insertions but also secondary genome alterations. HVO_1357 contains a domain that was previously identified in the regulation of bacteriorhodopsin transcription, as well as other domains frequently found in two-component regulatory systems. The genes adjacent to hvo_1357 encode a sensor box histidine kinase and a response regulator, key players of a two-component regulatory system. None of the homologues of HVO_2248 have been characterized, nor does it contain any of the assigned InterPro domains. However, in a significant number of Haloferax species, the adjacent gene codes for a chemotaxis receptor/transducer. Our results provide a foundation for characterizing the root causes underlying Hfx. volcanii hypermotility.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 12
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000610248000001
DOI: 10.3390/genes12010058
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Title: Genes
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI AG
Pages: - Volume / Issue: 12 (1) Sequence Number: 58 Start / End Page: - Identifier: ISSN: 2073-4425
CoNE: https://pure.mpg.de/cone/journals/resource/2073-4425