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  The GRIP1/14-3-3 pathway coordinates cargo trafficking and dendrite development

Geiger, J. C., Lipka, J., Segura, I., Hoyer, S., Schlager, M. A., Wulf, P. S., et al. (2014). The GRIP1/14-3-3 pathway coordinates cargo trafficking and dendrite development. Dev Cell, 28(4), 381-93. doi:10.1016/j.devcel.2014.01.018.

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Genre: Journal Article

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 Creators:
Geiger, J. C., Author
Lipka, J., Author
Segura, I., Author
Hoyer, S., Author
Schlager, M. A., Author
Wulf, P. S., Author
Weinges, S., Author
Demmers, J., Author
Hoogenraad, C. C., Author
Acker-Palmer, Amparo1, Author              
Affiliations:
1Neurovascular interface Group, Max Planck Institute for Brain Research, Max Planck Society, ou_2461707              

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Free keywords: 14-3-3 Proteins/*metabolism Adaptor Proteins, Signal Transducing/*metabolism Animals Carrier Proteins/genetics/metabolism Dendrites/*metabolism Gene Knockout Techniques/methods Kinesin/genetics/*metabolism Mice Mutation/genetics Nerve Tissue Proteins/genetics/*metabolism Protein Binding Protein Transport/genetics/physiology *Signal Transduction Transcription Factors/genetics/metabolism
 Abstract: Regulation of cargo transport via adaptor molecules is essential for neuronal development. However, the role of PDZ scaffolding proteins as adaptors in neuronal cargo trafficking is still poorly understood. Here, we show by genetic deletion in mice that the multi-PDZ domain scaffolding protein glutamate receptor interacting protein 1 (GRIP1) is required for dendrite development. We identify an interaction between GRIP1 and 14-3-3 proteins that is essential for the function of GRIP1 as an adaptor protein in dendritic cargo transport. Mechanistically, 14-3-3 binds to the kinesin-1 binding region in GRIP1 in a phospho-dependent manner and detaches GRIP1 from the kinesin-1 motor protein complex thereby regulating cargo transport. A single point mutation in the Thr956 of GRIP1 in transgenic mice impairs dendritic development. Together, our results show a regulatory role for GRIP1 during microtubule-based transport and suggest a crucial function for 14-3-3 proteins in controlling kinesin-1 motor attachment during neuronal development.

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 Dates: 2014-03-01
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: Other: 24576423
DOI: 10.1016/j.devcel.2014.01.018
ISSN: 1878-1551 (Electronic)1534-5807 (Linking)
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Title: Dev Cell
Source Genre: Journal
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Pages: - Volume / Issue: 28 (4) Sequence Number: - Start / End Page: 381 - 93 Identifier: -