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  Cone-shaped HIV-1 capsids are transported through intact nuclear pores

Zila, V., Margiotta, E., Turoňová, B., Müller, T. G., Zimmerli, C. E., Mattei, S., et al. (2021). Cone-shaped HIV-1 capsids are transported through intact nuclear pores. Cell, 184(4), 1032-1046. doi:10.1016/j.cell.2021.01.025.

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 Creators:
Zila, Vojtech1, Author
Margiotta, Erica2, 3, Author
Turoňová, Beata2, Author
Müller, Thorsten G.1, Author
Zimmerli, Christian E.2, Author
Mattei, Simone2, 4, Author
Allegretti, Matteo2, Author
Börner, Kathleen1, 5, Author
Rada, Jona1, Author
Müller, Barbara1, Author
Lusic, Marina1, 5, Author
Kräusslich, Hans-Georg1, 5, Author
Beck, Martin2, 6, Author              
Affiliations:
1Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany, ou_persistent22              
2European Molecular Biology Laboratory (EMBL), Heidelberg, Germany, ou_persistent22              
3Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, Heidelberg, Germany, ou_persistent22              
4Department of Biology, Institute of Molecular Biology and Biophysics, ETH Zurich, Zurich, Switzerland, ou_persistent22              
5German Center for Infection Research, partner site Heidelberg, Heidelberg, Germany, ou_persistent22              
6Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society, ou_3040395              

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Free keywords: capsid, correlative light and electron microscopy, cryoelectron tomography, electron tomography, human immunodeficiency virus, nuclear import, nuclear pore complex, uncoating
 Abstract: Human immunodeficiency virus (HIV-1) remains a major health threat. Viral capsid uncoating and nuclear import of the viral genome are critical for productive infection. The size of the HIV-1 capsid is generally believed to exceed the diameter of the nuclear pore complex (NPC), indicating that capsid uncoating has to occur prior to nuclear import. Here, we combined correlative light and electron microscopy with subtomogram averaging to capture the structural status of reverse transcription-competent HIV-1 complexes in infected T cells. We demonstrated that the diameter of the NPC in cellulo is sufficient for the import of apparently intact, cone-shaped capsids. Subsequent to nuclear import, we detected disrupted and empty capsid fragments, indicating that uncoating of the replication complex occurs by breaking the capsid open, and not by disassembly into individual subunits. Our data directly visualize a key step in HIV-1 replication and enhance our mechanistic understanding of the viral life cycle.

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Language(s): eng - English
 Dates: 2020-11-202020-07-302021-01-192021-02-102021-02-18
 Publication Status: Published in print
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.cell.2021.01.025
BibTex Citekey: zila_cone-shaped_2021
 Degree: -

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Title: Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 184 (4) Sequence Number: - Start / End Page: 1032 - 1046 Identifier: ISSN: 0092-8674
CoNE: https://pure.mpg.de/cone/journals/resource/954925463183