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  X-ray Emission Spectroscopy Evidences a Central Carbon in the Nitrogenase Iron-Molybdenum Cofactor

Lancaster, K. M., Roemelt, M., Ettenhuber, P., Hu, Y., Ribbe, M. W., Neese, F., et al. (2011). X-ray Emission Spectroscopy Evidences a Central Carbon in the Nitrogenase Iron-Molybdenum Cofactor. Science, 334(6058), 974-977. doi:10.1126/science.1206445.

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Lancaster, Kyle M.1, Author
Roemelt, Michael2, Author              
Ettenhuber, Patrick2, Author
Hu, Yilin3, Author
Ribbe, Markus W.3, Author
Neese, Frank2, 4, Author              
Bergmann, Uwe5, Author
DeBeer, Serena1, 4, Author              
Affiliations:
1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA, ou_persistent22              
2Institut für Physikalische und Theoretische Chemie, Universität Bonn, Wegelerstr. 12, D-53115 Bonn, Germany, ou_persistent22              
3Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA, ou_persistent22              
4Research Department Neese, Max Planck Institute for Bioinorganic Chemistry, Max Planck Society, ou_3023879              
5Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA, ou_persistent22              

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 Abstract: Nitrogenase is a complex enzyme that catalyzes the reduction of dinitrogen to ammonia. Despite insight from structural and biochemical studies, its structure and mechanism await full characterization. An iron-molybdenum cofactor (FeMoco) is thought to be the site of dinitrogen reduction, but the identity of a central atom in this cofactor remains unknown. Fe Kβ x-ray emission spectroscopy (XES) of intact nitrogenase MoFe protein, isolated FeMoco, and the FeMoco-deficient ∆nifB protein indicates that among the candidate atoms oxygen, nitrogen, and carbon, it is carbon that best fits the XES data. The experimental XES is supported by computational efforts, which show that oxidation and spin states do not affect the assignment of the central atom to C4–. Identification of the central atom will drive further studies on its role in catalysis.

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Language(s): eng - English
 Dates: 2011-11-18
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/science.1206445
 Degree: -

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Title: Science
  Abbreviation : Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 334 (6058) Sequence Number: - Start / End Page: 974 - 977 Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1