English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  High-Pressure Stopped-Flow Spectrometer for Kinetic Studies of Fast Reactions by Absorbance and Fluorescence Detection

Bugnon, P., Laurenczy, G., Ducommun, Y., Sauvageat, P.-Y., Merbach, A. E., Ith, R., et al. (1996). High-Pressure Stopped-Flow Spectrometer for Kinetic Studies of Fast Reactions by Absorbance and Fluorescence Detection. Analytical Chemistry, 68(17), 3045-3049. doi:10.1021/ac960382k.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Bugnon, Pascal1, Author
Laurenczy, Gábor 1, Author
Ducommun, Yves1, Author
Sauvageat, Pierre-Yves1, Author
Merbach, André E. 1, Author
Ith, Roger2, Author
Tschanz, René 2, Author
Doludda, Michael3, Author           
Bergbauer, Rolf3, Author           
Grell, Ernst3, Author           
Affiliations:
1Institut de Chimie Minérale et Analytique, Université de Lausanne, BCH, CH-1015 Lausanne, Switzerland , ou_persistent22              
2Atelier Mécanique, Section de Chimie, Université de Lausanne, BCH, CH-1015 Lausanne, Switzerland , ou_persistent22              
3Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              

Content

show
hide
Free keywords: Reagents; Fluorescence detection; Absorption; Fluorescence; Light
 Abstract: The development of a stopped-flow instrument that operates over a temperature range of −40 to +100 °C and up to 200 MPa is described. The system has been designed so that measurements can be performed in absorbance and fluorescence modes simultaneously, without dismantling the unit. It can easily be combined with an optical system of a conventional ambient pressure setup by using light guides. Optimum optical performance and a wide operating wavelength range (220−850 nm) are achieved as the light is not passing through the pressurizing fluid. A special design for the pistons has been developed; thus, the apparatus has proven to be leak-free, even under extreme conditions (high pressure, low temperature, various solvents). The dead time of the system is found to be less than 2 ms at 298 K and is pressure independent up to 200 MPa. We examined the kinetics for the formation of the Mg2+−8-hydroxyquinoline chelate in aqueous solutions at pH 8.0 in order to develop a convenient alternative test method for high-pressure stopped-flow spectrometers with absorption and fluorescence detection.

Details

show
hide
Language(s): eng - English
 Dates: 1996-04-181996-05-291996-09-011996-09-01
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/ac960382k
PMID: 21619372
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Analytical Chemistry
  Abbreviation : Anal. Chem.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 68 (17) Sequence Number: - Start / End Page: 3045 - 3049 Identifier: ISSN: 0003-2700
CoNE: https://pure.mpg.de/cone/journals/resource/111032812862552