Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Amiloride-inhibitable Na+ conductance in rat proximal tubule

Willman, J., Bleich, M., Rizzo, M., Schmidt-Hieber, M., Ullrich, K. J., & Greger, R. (1997). Amiloride-inhibitable Na+ conductance in rat proximal tubule. Pflügers Archiv: European Journal of Physiology, 434(2), 173-178. doi:10.1007/s004240050380.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Willman, J.K.1, Autor
Bleich, M.1, Autor
Rizzo, M.1, Autor
Schmidt-Hieber, M.1, Autor
Ullrich, Karl Julius2, Autor           
Greger, Rainer1, Autor
Affiliations:
1Physiologisches Institut der Albert-Ludwigs-Universitat, Hermann-Herder-Strasse 7, D-79104 Freiburg i. Br., Germany, ou_persistent22              
2Emeritusgroup Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_3273468              

Inhalt

einblenden:
ausblenden:
Schlagwörter: Na+ channel; Epithelial Na+ channel; Na+ absorption; Na+ deprivation; Patch-clamp technique
 Zusammenfassung: Previous single-channel recordings from the luminal membrane of the rabbit proximal tubule have revealed amiloride-inhibitable Na+ channels of a characteristic conductance range. The present study aimed to pursue this issue in rat proximal tubule. Control rats were compared to those put on a low-Na+ diet or pretreated by triamcinolone injections (s.c.). Stimulation of Na+ absorption by glucocorticoids was verified by examining the transepithelial voltage in Ussing chamber studies of the distal colon. The membrane voltage (Vm) of isolated, in-vitro-perfused proximal tubule segments was measured in patch-clamp and impalement studies. It was found that amiloride hyperpolarized Vm significantly by 2.1 ± 0.9 mV (n = 26) in tubules of control rats, by 3.9 ± 0.7 mV (n = 12) in rats put on a low-Na+ diet and by 3.7 ± 1.0 mV (n = 17) in rats treated with glucocorticoids. The effect of amiloride was concentration dependent with a half-maximal effect at < 1 micromol/l. RT-PCR techniques were used to search for the presence of the alpha-, beta- and gamma-subunits of the epithelial Na+ channel in isolated proximal tubule segments. The presence of the respective mRNAs was verified. These data indicate that: (1) amiloride-inhibitable Na+ channels are present in rate proximal tubules; (2) the Na+ conductance may be up-regulated by Na+ deprivation but is still very limited when compared to total cell conductance; (3) therefore, the contribution of Na+-channel-mediated absorption to total proximal Na+ absorption is probably small.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 1997-01-221996-08-051997-01-281997-05-01
 Publikationsstatus: Erschienen
 Seiten: 6
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1007/s004240050380
PMID: 9136671
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Pflügers Archiv: European Journal of Physiology
  Andere : Pflügers Arch. Europ. J. Physiol.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Heidelberg : Springer-Verlag
Seiten: - Band / Heft: 434 (2) Artikelnummer: - Start- / Endseite: 173 - 178 Identifikator: ISSN: 0031-6768
CoNE: https://pure.mpg.de/cone/journals/resource/954925432380