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  Dynamic laser light scattering to determine size distributions of vesicles

Ruf, H., Georgalis, Y., & Grell, E. (1989). Dynamic laser light scattering to determine size distributions of vesicles. In S. Fleischer, & B. Fleischer (Eds.), Methods in Enzymology (pp. 364-390). San Diego, California 92101, USA: Academic Press. doi:10.1016/s0076-6879(89)72024-3.

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 Urheber:
Ruf, Horst1, Autor           
Georgalis, Yannis2, Autor
Grell, Ernst1, Autor           
Affiliations:
1Molecular Biophysics Group, Max Planck Institute of Biophysics, Max Planck Society, ou_3264820              
2Abteilung Saenger, Institut für Kristallographie der Freien Universität Berlin, Germany, ou_persistent22              

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 Zusammenfassung: Publisher Summary:
This chapter describes dynamic laser light scattering to determine size distributions of vesicles and presents a survey of the underlying principles with regard to vesicle suspensions. From a size distribution, it can be decided whether vesicles are homogeneous in size. Its characteristics can be used for controlling the stability of a sample and for studying processes such as aggregation and fusion. The nonmicroscopic methods requiring rather homogeneous populations with a well-defined shape are most appropriately applied to small unilamellar vesicles. Electron microscopy is used for vesicles having an extended size range. Because of the remarkable sensitivity of parameters to the size distribution of vesicles based on the composition of a medium, it is important to characterize and analyze each vesicle preparation.

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Sprache(n): eng - English
 Datum: 2004-01-071989
 Publikationsstatus: Erschienen
 Seiten: 5
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1016/s0076-6879(89)72024-3
PMID: 2747535
 Art des Abschluß: -

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Titel: Methods in Enzymology
  Untertitel : Biomembranes Part S Transport: Membrane Isolation and Characterization
Genre der Quelle: Reihe
 Urheber:
Fleischer, Sidney1, Herausgeber
Fleischer, Becca1, Herausgeber
Affiliations:
1 Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee, USA, ou_persistent22            
Ort, Verlag, Ausgabe: San Diego, California 92101, USA : Academic Press
Seiten: 776 Band / Heft: 172 Artikelnummer: - Start- / Endseite: 364 - 390 Identifikator: ISBN: 978-0-12-182073-4
ISSN: 0076-6879