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  Dynamic laser light scattering to determine size distributions of vesicles

Ruf, H., Georgalis, Y., & Grell, E. (1989). Dynamic laser light scattering to determine size distributions of vesicles. In S. Fleischer, & B. Fleischer (Eds.), Methods in Enzymology (pp. 364-390). San Diego, California 92101, USA: Academic Press. doi:10.1016/s0076-6879(89)72024-3.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-00BF-1 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-00C0-E
Genre: Book Chapter

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 Creators:
Ruf, Horst1, Author           
Georgalis, Yannis2, Author
Grell, Ernst1, Author           
Affiliations:
1Molecular Biophysics Group, Max Planck Institute of Biophysics, Max Planck Society, ou_3264820              
2Abteilung Saenger, Institut für Kristallographie der Freien Universität Berlin, Germany, ou_persistent22              

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 Abstract: Publisher Summary:
This chapter describes dynamic laser light scattering to determine size distributions of vesicles and presents a survey of the underlying principles with regard to vesicle suspensions. From a size distribution, it can be decided whether vesicles are homogeneous in size. Its characteristics can be used for controlling the stability of a sample and for studying processes such as aggregation and fusion. The nonmicroscopic methods requiring rather homogeneous populations with a well-defined shape are most appropriately applied to small unilamellar vesicles. Electron microscopy is used for vesicles having an extended size range. Because of the remarkable sensitivity of parameters to the size distribution of vesicles based on the composition of a medium, it is important to characterize and analyze each vesicle preparation.

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Language(s): eng - English
 Dates: 2004-01-071989
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/s0076-6879(89)72024-3
PMID: 2747535
 Degree: -

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Title: Methods in Enzymology
  Subtitle : Biomembranes Part S Transport: Membrane Isolation and Characterization
Source Genre: Series
 Creator(s):
Fleischer, Sidney1, Editor
Fleischer, Becca1, Editor
Affiliations:
1 Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee, USA, ou_persistent22            
Publ. Info: San Diego, California 92101, USA : Academic Press
Pages: 776 Volume / Issue: 172 Sequence Number: - Start / End Page: 364 - 390 Identifier: ISBN: 978-0-12-182073-4
ISSN: 0076-6879