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Free keywords:
Alkali Ion Selectivity; Fluorescence; Na,K‐ATPase; Stability Constants; Thermodynamics; Titrations
Abstract:
In order to investigate the high‐affinity K+ binding to proteins, alkali ion binding studies were performed on a fluorescent derivative of membrane‐bound Na,K‐ATPase in buffered aqueous solution at 37°C employing a specially designed automatic spectrofluorometric titration system. The comparison of the obtained apparent stability constants, which reached values for K+ even over 104 M−1 with those of a simpler cyclic peptide, acting as a suitable model compound, provides suggestions concerning the molecular nature of the binding sites involved.
