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  Nuclear envelope permeability measured by fluorescence microphotolysis of single liver cell nuclei

Peters, R. (1983). Nuclear envelope permeability measured by fluorescence microphotolysis of single liver cell nuclei. The Journal of Biological Chemistry, 258(19), 11427-11429. doi:10.1016/S0021-9258(17)44241-4.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-052B-3 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-052C-2
Genre: Journal Article

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 Creators:
Peters, Reiner1, Author           
Affiliations:
1Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_3264817              

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 Abstract: Fluorescence microphotolysis ("photobleaching") has been widely used to measure translational diffusion coefficients of lipids and proteins in cell membranes. This communication shows that fluorescence microphotolysis can be also employed for measurement of membrane transport in single cells and organelles. The influx of fluorescently labeled dextrans of graded molecular size into leaky human erythrocyte ghosts and isolated rat liver cell nuclei has been measured. For the nuclear envelope, a functional pore radius of 56-59 A is derived.

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Language(s): eng - English
 Dates: 1983-06-292021-01-041983-10-10
 Publication Status: Issued
 Pages: 3
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/S0021-9258(17)44241-4
PMID: 6194152
 Degree: -

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Title: The Journal of Biological Chemistry
  Other : JBC
  Abbreviation : J. Biol. Chem.
Source Genre: Journal
 Creator(s):
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Publ. Info: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Pages: - Volume / Issue: 258 (19) Sequence Number: - Start / End Page: 11427 - 11429 Identifier: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826_1