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Abstract:
Fluorescence microphotolysis (‘photobleaching’) has been widely used for studying the translational mobility of membrane proteins and lipids. Recently, the technique has been used for measuring, in single normal-sized cells, the transport of solutes through membranes. Such single-cell flux measurements could not be performed previously and open new possibilities to membrane transport research. This article discusses the methodological basis of fluorescence microphotolysis and two applications: anion transport in red cell ghosts and nucleocytoplasmic transport in cultured liver cells.
