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  Identification of the D-glucose binding polypeptide of the renal Na+-D-glucose cotransporter with a covalently binding D-glucose analog

Neeb, M., Fasold, H., & Koepsell, H. (1985). Identification of the D-glucose binding polypeptide of the renal Na+-D-glucose cotransporter with a covalently binding D-glucose analog. FEBS Letters, 182(1), 139-144. doi:10.1016/0014-5793(85)81171-6.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-053C-0 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-053D-F
Genre: Journal Article

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 Creators:
Neeb, Martin1, Author           
Fasold, Hugo2, Author
Koepsell, Hermann1, Author           
Affiliations:
1Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              
2Institut für Biochemie, Johann Wolfgang Goethe-Universität, Frankfurt, FRG, ou_persistent22              

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Free keywords: Affinity labeling; D-Glucose analog; D-Glucose binding site; Na+-D-glucose cotransporter; Brush-border membrane; Kidney
 Abstract: The covalently binding D-glucose analog 10-N-(bromoacetyl)amino-1-decyl-beta-D-glucopyranoside (BADG) was synthesised and shown to be a high-affinity inhibitor of the renal Na+-D-glucose contransporter. From renal brush-border membranes a protein fraction was isolated, in which the concentration of Na+-dependent phlorizin binding sites per mg protein was enriched 7-fold. In labeling experiments with this protein fraction a polypeptide of Mr approximately 79000 was identified as containing the D-glucose binding site of the renal Na+-D-glucose cotransporter.

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Language(s): eng - English
 Dates: 1985-01-042001-11-121985-03-11
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0014-5793(85)81171-6
PMID: 3838282
 Degree: -

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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 182 (1) Sequence Number: - Start / End Page: 139 - 144 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501