Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

 
 
DownloadE-Mail
  Role of clathrin light chains in regulating invadopodia formation

Mukenhirn, M., Muraca, F., Bucher, D., Asberger, E., Barazzone, E. C., Cavalcanti-Adam, E. A., et al. (2021). Role of clathrin light chains in regulating invadopodia formation. Cells, 10: 451, pp. 1-26. doi:10.3390/cells10020451.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
Cells_10_2021_451.pdf (beliebiger Volltext), 5MB
 
Datei-Permalink:
-
Name:
Cells_10_2021_451.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Medical Research, MHMF; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
Cells_10_2021_451_Suppl.zip (Ergänzendes Material), 27MB
 
Datei-Permalink:
-
Name:
Cells_10_2021_451_Suppl.zip
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Medical Research, MHMF; )
MIME-Typ / Prüfsumme:
application/zip
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:
ausblenden:
externe Referenz:
https://www.mdpi.com/2073-4409/10/2/451/pdf (beliebiger Volltext)
Beschreibung:
-
OA-Status:
externe Referenz:
https://doi.org/10.3390/cells10020451 (beliebiger Volltext)
Beschreibung:
-
OA-Status:
externe Referenz:
https://www.mdpi.com/2073-4409/10/2/451/s1 (Ergänzendes Material)
Beschreibung:
-
OA-Status:

Urheber

einblenden:
ausblenden:
 Urheber:
Mukenhirn, Markus, Autor
Muraca, Francesco, Autor
Bucher, Delia, Autor
Asberger, Edgar, Autor
Barazzone, Elisa Cappio, Autor
Cavalcanti-Adam, Elisabetta Ada1, Autor           
Boulant, Steeve, Autor
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

Inhalt

einblenden:
ausblenden:
Schlagwörter: clathrin light chain; invadopodia; MMP14: actin; membrane trafficking
 Zusammenfassung: One of the most fundamental processes of the cell is the uptake of molecules from the surrounding environment. Clathrin-mediated endocytosis (CME) is the best-described uptake pathway and regulates nutrient uptake, protein and lipid turnover at the plasma membrane (PM), cell signaling, cell motility and cell polarity. The main protein in CME is clathrin, which assembles as a triskelion-looking building block made of three clathrin heavy chains and three clathrin light chains. Compared to clathrin heavy chains (CHCs), the role of the two isoforms of clathrin light chains (CLCA and CLCB) is poorly understood. Here, we confirm that the simultaneous deletion of both CLCA/B causes abnormal actin structures at the ventral PM and we describe them, for the first time, as functional invadopodia rather than disorganized actin-cytoskeleton assembly sites. Their identification is based on the occurrence of common invadopodia markers as well as functional invadopodia activity characterized by an increased local proteolytic activity of the extracellular matrix proteins. We demonstrate that CLCA/B deletion impacts the intracellular trafficking and recovery of the matrix metalloproteinase 14 (MMP14) leading to its accumulation at the plasma membrane and induction of invadopodia formation. Importantly, we show that invadopodia formation can be prevented by depletion of MMP14. As such, we propose that CLCA/B regulate invadopodia formation by regulating MMP14 delivery to the plasma membrane.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2021-02-022020-12-202021-02-172021-02-20
 Publikationsstatus: Online veröffentlicht
 Seiten: 26
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.3390/cells10020451
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Cells
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Basel, Switzerland : MDPI
Seiten: - Band / Heft: 10 Artikelnummer: 451 Start- / Endseite: 1 - 26 Identifikator: CoNE: https://pure.mpg.de/cone/journals/resource/2073-4409