English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Unravelling the structural complexity of glycolipids with cryogenic infrared spectroscopy

Kirschbaum, C., Greis, K., Mucha, E., Kain, L., Deng, S., Zappe, A., et al. (2021). Unravelling the structural complexity of glycolipids with cryogenic infrared spectroscopy. Nature Communications, 12: 1201. doi:10.1038/s41467-021-21480-1.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
s41467-021-21480-1.pdf (Publisher version), 929KB
Name:
s41467-021-21480-1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
2021
Copyright Info:
The Author(s)

Locators

show

Creators

show
hide
 Creators:
Kirschbaum, Carla1, 2, Author              
Greis, Kim1, 2, Author              
Mucha, Eike2, Author              
Kain, Lisa3, Author
Deng, Shenglou4, Author
Zappe, Andreas1, Author
Gewinner, Sandy2, Author              
Schöllkopf, Wieland2, Author              
Helden, Gert von2, Author              
Meijer, Gerard2, Author              
Savage, Paul B.4, Author
Marianski, Mateusz5, Author
Teyton, Luc3, Author
Pagel, Kevin1, 2, Author              
Affiliations:
1Institut für Chemie und Biochemie, Freie Universität Berlin, Berlin, Germany, ou_persistent22              
2Molecular Physics, Fritz Haber Institute, Max Planck Society, ou_634545              
3Department of Immunology and Microbiology, Scripps Research, La Jolla, CA, USA, ou_persistent22              
4Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA, ou_persistent22              
5Department of Chemistry and Biochemistry, Hunter College, The City University of New York, New York, NY, USA, ou_persistent22              

Content

show
hide
Free keywords: Glycobiology; Glycolipids; Immunology; Infrared spectroscopy
 Abstract: Glycolipids are complex glycoconjugates composed of a glycan headgroup and a lipid moiety. Their modular biosynthesis creates a vast amount of diverse and often isomeric structures, which fulfill highly specific biological functions. To date, no gold-standard analytical technique can provide a comprehensive structural elucidation of complex glycolipids, and insufficient tools for isomer distinction can lead to wrong assignments. Herein we use cryogenic gas-phase infrared spectroscopy to systematically investigate different kinds of isomerism in immunologically relevant glycolipids. We show that all structural features, including isomeric glycan headgroups, anomeric configurations and different lipid moieties, can be unambiguously resolved by diagnostic spectroscopic fingerprints in a narrow spectral range. The results allow for the characterization of isomeric glycolipid mixtures and biological applications.

Details

show
hide
Language(s): eng - English
 Dates: 2020-05-292021-01-262021-02-22
 Publication Status: Published online
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-021-21480-1
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: 7 Volume / Issue: 12 Sequence Number: 1201 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723