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  Translation and replication of hepatitis C virus genomic RNA depends on ancient cellular proteins that control mRNA fates

Scheller, N., Mina, L. B., Galão, R. P., Chari, A., Giménez-Barcons, M., Noueiry, A., et al. (2009). Translation and replication of hepatitis C virus genomic RNA depends on ancient cellular proteins that control mRNA fates. Proceedings of the National Academy of Sciences of the USA, 106(32), 13517-13522. doi:10.1073/pnas.0906413106.

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3287423.pdf (Publisher version), 933KB
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 Creators:
Scheller, N., Author
Mina, L. B., Author
Galão, R. P., Author
Chari, A.1, Author           
Giménez-Barcons, M., Author
Noueiry, A., Author
Fischer, U., Author
Meyerhans, A., Author
Díez, J., Author
Affiliations:
1Research Group of Structural Biochemistry and Mechanisms, MPI for Biophysical Chemistry, Max Planck Society, ou_3265855              

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Free keywords: deadenylation-dependent mRNA decay, HCV, host factors, LSm1–7, Rck/p54
 Abstract: Inevitably, viruses depend on host factors for their multiplication. Here, we show that hepatitis C virus (HCV) RNA translation and replication depends on Rck/p54, LSm1, and PatL1, which regulate the fate of cellular mRNAs from translation to degradation in the 5′-3′-deadenylation-dependent mRNA decay pathway. The requirement of these proteins for efficient HCV RNA translation was linked to the 5′ and 3′ untranslated regions (UTRs) of the viral genome. Furthermore, LSm1–7 complexes specifically interacted with essential cis-acting HCV RNA elements located in the UTRs. These results bridge HCV life cycle requirements and highly conserved host proteins of cellular mRNA decay. The previously described role of these proteins in the replication of 2 other positive-strand RNA viruses, the plant brome mosaic virus and the bacteriophage Qß, pinpoint a weak spot that may be exploited to generate broad-spectrum antiviral drugs.

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Language(s): eng - English
 Dates: 2009-07-232009-08-11
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1073/pnas.0906413106
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Title: Proceedings of the National Academy of Sciences of the USA
Source Genre: Journal
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Pages: - Volume / Issue: 106 (32) Sequence Number: - Start / End Page: 13517 - 13522 Identifier: -