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  LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication

Galão, R. P., Chari, A., Alves-Rodrigues, I., Lobão, D., Mas, A., Kambach, C., et al. (2010). LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication. RNA, 16(4), 817-827. doi:10.1261/rna.1712910.

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3287428.pdf (Publisher version), 898KB
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 Creators:
Galão, R. P., Author
Chari, A.1, Author           
Alves-Rodrigues, I., Author
Lobão, D., Author
Mas, A., Author
Kambach, C., Author
Fischer, U., Author
Díez, J., Author
Affiliations:
1Research Group of Structural Biochemistry and Mechanisms, MPI for Biophysical Chemistry, Max Planck Society, ou_3265855              

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Free keywords: BMV, LSm1-7, RNA virus, Sm proteins, translation, replication
 Abstract: LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3′-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that these sequences regulate the translation and replication of the BMV genome. Furthermore, both RNA-target sequences resemble those found for Hfq, the LSm counterpart in bacteria, suggesting conservation through evolution. Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions.

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Language(s): eng - English
 Dates: 2010-02-242010-04
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1261/rna.1712910
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Title: RNA
Source Genre: Journal
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Pages: - Volume / Issue: 16 (4) Sequence Number: - Start / End Page: 817 - 827 Identifier: -