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  Nanoindentation of mesenchymal stem cells using atomic force microscopy: effect of adhesive cell-substrate structures

Migliorini, E., Cavalcanti-Adam, E. A., Uva, A. E., Fiorentino, M., Gattullo, M., Manghisi, V. M., et al. (2021). Nanoindentation of mesenchymal stem cells using atomic force microscopy: effect of adhesive cell-substrate structures. Nanotechnology, 32(21): 215706, pp. 1-16. doi:10.1088/1361-6528/abe748.

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 Creators:
Migliorini, Elisa, Author
Cavalcanti-Adam, Elisabetta Ada1, Author              
Uva, Antonio Emmanuele, Author
Fiorentino, Michele, Author
Gattullo, Michele, Author
Manghisi, Vito Modesto, Author
Vaiani, Lorenzo, Author
Boccaccio, Antonio, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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 Abstract: The procedure commonly adopted to characterize cell materials using atomic force microscopy neglects the stress state induced in the cell by the adhesion structures that anchor it to the substrate. In several studies, the cell is considered as made from a single material and no specific information is provided regarding the mechanical properties of subcellular components. Here we present an optimization algorithm to determine separately the material properties of subcellular components of mesenchymal stem cells subjected to nanoindentation measurements. We assess how these properties change if the adhesion structures at the cell-substrate interface are considered or not in the algorithm. In particular, among the adhesion structures, the focal adhesions and the stress fibers were simulated. We found that neglecting the adhesion structures leads to underestimate the cell mechanical properties thus making errors up to 15 %. This result leads us to conclude that the action of adhesion structures should be taken into account in nanoindentation measurements especially for cells that include a large number of adhesions to the substrate.

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Language(s): eng - English
 Dates: 2021-02-172021-02-172021-03-05
 Publication Status: Published in print
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1088/1361-6528/abe748
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Title: Nanotechnology
  Abbreviation : Nanotechnology
Source Genre: Journal
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Publ. Info: Bristol, UK : IOP Pub.
Pages: - Volume / Issue: 32 (21) Sequence Number: 215706 Start / End Page: 1 - 16 Identifier: ISSN: 0957-4484
CoNE: https://pure.mpg.de/cone/journals/resource/954925577042