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  Conversion of the myometrial oxytocin receptor from low to high affinity state by cholesterol

Fahrenholz, F., Klein, U., & Gimpl, G. (1996). Conversion of the myometrial oxytocin receptor from low to high affinity state by cholesterol. Springer; 1. Edition.

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Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-0008-0ED4-A Versions-Permalink: https://hdl.handle.net/21.11116/0000-0008-0ED5-9
Genre: Konferenzbeitrag

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Urheber

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 Urheber:
Fahrenholz, Falk1, Autor           
Klein, Uwe1, Autor           
Gimpl, Gerald1, Autor           
Affiliations:
1Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

Inhalt

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Schlagwörter: -
 Zusammenfassung: Reconstitution experiments with the myometrial oxytocin receptor from guinea pig showed a strong requirement of the oxytocin receptor binding function for the presence of cholesterol in preformed liposomes. To investigate the effect of cholesterol on the oxytocin receptor in the plasma membrane, a new method to modify the membrane cholesterol content was developed. With substituted cyclodextrins we were able to selectively deplete the myometrial plasma membrane of cholesterol. Vice versa, incubation of cholesterol-depleted membranes with a soluble cholesterol cyclodextrin complex restored the cholesterol content of the plasma membrane. Binding experiments showed, that with the removal of cholesterol from the membrane, the oxytocin receptor was converted from high to low affinity state. Increasing the cholesterol content of the membrane again restored the high binding affinity. Substitution of membrane cholesterol with other steroids showed a strong dependence of the oxytocin receptor function on the structure of the cholesterol molecule. Experiments with the detergent solubilized oxytocin receptor and with oxytocin receptors expressed in the Baculovirus/Sf9 cell system provided further evidence for a direct interaction of the oxytocin receptor with cholesterol.

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Sprache(n): eng - English
 Datum: 19951996-03-31
 Publikationsstatus: Erschienen
 Seiten: 9
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: PMID: 8713981
 Art des Abschluß: -

Veranstaltung

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Titel: Proceedings of the 3rd Hanseatic Endocrine Conference on Oxytocin
Veranstaltungsort: Stade, Germany
Start-/Enddatum: 1995-04-30 - 1995-05-04

Entscheidung

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Quelle 1

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Titel: Advances in Experimental Medicine and Biology
  Untertitel : Oxytocin, cellular and molecular approaches in medicine and research
Genre der Quelle: Reihe
 Urheber:
Ivell, Richard1, Herausgeber
Russell, John, Autor
Affiliations:
1 Institute for Hormone and Fertility Research, University of Hamburg, Germany, ou_persistent22            
Ort, Verlag, Ausgabe: Springer; 1. Edition
Seiten: 692 Band / Heft: 395 Artikelnummer: - Start- / Endseite: 311 - 319 Identifikator: ISBN: 978-0306452901