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Free keywords:
ADENYLYLATION; TARGETSBiochemistry & Molecular Biology; Pharmacology & Pharmacy; AMPylation; click chemistry; fluorescent probes; post-translational
modifications; proteomics;
Abstract:
Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide-alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.
